Effect of Tongxinluo capsule combined with stem cell transplantation on promoting angiogenesis in diabetic foot rats
LI Xiaolin
GAO Huailin
LI Bing
WANG Hairong
LIU Huan
WANG Sheng.
Abstract：Objective To discuss promoting angiogenesis by observing the levels of vascular endothelial growth factor (VEGF),advanced glycation end products(AGE),advanced glycation end products receptor(RAGE) in the diabetic foot ul-cers rats treated by Tongxinluo capsule combined with peripheral blood stem cell transplantation. Methods From October 2012 to August 2013,the research was conducted in Hebei Yiling Medicine Pharmacology Laboratory. The diabetic rat model was es-tablished. Diabetic foot ulcers rats were randomly divided into five groups(n=10):normal control,model,TXL,stem cell, TXL + stem cells,the serum level of VEGF,AGE,RAGE were determined by enzyme linked immunosorbent assay,the local organizations levels of VEGF mRNA,RAGE mRNA expression were examined by RT PCR method. Results The comparison between groups of serum VEGF,AGE and RAGE have significant difference (F=27.675,F=18.018,F=35.946,P=0. 000),compared with model group, Tongxinluo capsule group and Tongxinluo capsule + stem cell group serum VEGF in-creased significantly (P=0.003, P=0.000), AGE, RAGE decreased significantly (P=0.006, P=0.000), stem cell group increased(P=0.015),AGE and RAGE decreased(P=0.036,P=0.021);Tongxinluo capsule group showed signif-icantly increased serum VEGF (P=0.006), AGE and RAGE decreased (P=0.017, P=0.030), Tongxinluo capsule +stem cell group's serum VEGF increased significantly (P=0.000),AGE,RAGE significantly decreased (P=0.002, P=0.000);compared with Tongxinluo capsule group,Tongxinluo capsule + stem cell group showed increased of serum VEGF (P=0.041), AGE and RAGE decreased (P =0.035, P =0.013); Among the groups, ulcer tissue VEGF mRNA and RAGE mRNA expression have significant difference (F=8.152,F=7.650, P=0.000), compared with the model group, Tongxinluo capsule group increased the expression of VEGF mRNA and decreased the expression of RAGE mRNA (P=0. 014,P=0.023), Tongxinluo capsule + stem cells group significantly increased the expression of VEGF mRNA and de-creased the expression of RAGE mRNA (P=0.003, P=0.000); in stem cell group, the expression of RAGE mRNA and VEGF mRNA in ulcer tissue showed no significant difference compared with the model group(P=0.235,P=0.470);com-pared with the stem cell group,Tongxinluo capsule group significantly increased the expression of VEGF mRNA in local ulcer (P=0.008) and decreased the expression of RAGE mRNA (P=0.039), Tongxinluo capsule + stem cells group signifi-cantly with increased local VEGF mRNA expression(P=0.002),significantly reduced the expression of RAGE mRNA(P=0.001); compared with Tongxinluo capsule group, Tongxinluo capsule + stem cell group increased ulcer's expression of VEGF mRNA (P=0.032),reduced the expression of RAGE mRNA (P=0.027). Conclusion Tongxinluo capsule com-bined peripheral blood stem cell transplantation can improve the VEGF levels in serum and local organizations of diabetic foot ulcers rats,and reduce AGE,RAGE levels,promoting angiogenesis.
Keywords：Diabetic footTongxinluo capsuleStem cell transplantationVascular endothelial growth factorAd-vanced glycation end productsRats
Publication Date：2018-01-01
Pages：5( 272-276 )