Effect of angiotensinⅡand β aminopropionitrile monofumarate to induce abdominal aortic aneurysm models in mice

SUN Jingyuan; GAO Ling; YE Yingchun; LUO Deng; YANG Heng

doi:10.3969/j.issn.1671-6450.2018.03.012

Abstract：Objective To investigate the characteristic and feasibility of mice model of abdominal aortic aneurysm (AAA) induced by AngiotensinⅡ (AngⅡ) combining with β aminopropionitrile monofumarate(BAPN). Methods Thirty C57BL6 mice were randomly divided into control group,model A group and model B group,with 10 mice in each group. The model A group was subcutaneous infused with AngⅡthrough the implantation of osmotic pumps for 28 days at a rate of 1 μg· kg-1·min-1,meanwhile being fed with a diet containing 0.25% BAPN for 28 days. The model B group was fed with a diet containing 0.25% BAPN for 27 days,followed by AngⅡinfusion for 24h at a rate of 1 μg·kg-1·min-1. The control group was infused with saline and fed with a chow diet. The incidence of AAA,diameter of abdominal aortic,ROS staining and elas-tin staining,the physiopathologic changes of the abdominal aorta were compared between model groups and the control group. The expression of MMP-2 was detected with immunohistochemical staining. Results The incidence of AAA in model A group and model B group was 70.0% and 22.2%, respectively. Diameter of abdominal aortic in model A group was greater com-pared with the control group(P<0.01),while the comparison between model B group and the control group was not statisti-cally significant. And diameter of abdominal aortic in model A group was greater compared with model B group (P<0.05). ROS staining showed that the expression was up regulated in model groups compared with the control group. Elastin staining showed that elastic fibers were degraded and disordered in model groups. Immunohistochemical staining showed that the ex-pression of MMP 2 increased in model groups compared with the control group. Conclusion The AAA model could be suc-cessfully established with Ang Ⅱand BAPN.