The efficacy of taurine on microglial activation in ischemic brain tissue of rats during cerebral ischemia reperfusion damage

WANG Lijuan; ZHAO Liuzhuang; ZHOU Xiaomei; NIU Junwei

doi:10.3969/j.issn.1671-6450.2018.01.018

Abstract：Objective To investigate whether taurine could reduce microglial activation and inhibit inflammatory to protect brain tissue against cerebral ischemia reperfusion damage.Methods The experiment was carried out in Capital Medical University Beijing Neurosurgery Research Institute From Mar.2016 to May 2017.6 rats of 54 were used to prepare emboli,other 48 rats was randomly divided into 4 groups with 12 rats in each group:2 h ischemia following 4 h reperfusion control group Ⅰ,2 h ischemia following 4h reperfusion administration group Ⅰ,2 h ischemia following 22 h reperfusion control group Ⅱ,2 h ischemia following 22h reperfusion administration group Ⅱ.Thromboembolic model of transient focal cerebral ischemia reperfusion was induced in the 48 rats.The efficacy of taurine on microglial activation at 2 hours ischemia following 4 and 22 hours of reperfusion was evaluated by major histocompatibility complex (MHC) antigen immunohistochemistry.Results Compared with control group Ⅰ,administration group Ⅰ could reduce numerical density of the MHC class Ⅰ immunopositive microglia in ischemic gray matter,corpus callosum and caudateputamen (t MHC Ⅰ =3.594,5.169,4.269,P ＜ 0.01;t MHCⅡ =3.511,3.087,4.743,P ＜0.05).Compared with control group Ⅱ,administration group Ⅱ could reduce numerical density of the MHC class Ⅰ immunopositive microglia in ischemic gray matter,corpus callosum and caudate putamen(tMHCⅠ =11.691,13.262,6.070,1.914,P ＜0.01;tMHCⅡ =30.533,38.554,5.913,2.311,P ＜0.05).Taurine could reduce numerical density of the MHC class Ⅰ and class Ⅱ immunopositive microglia in ischemic gray matter and white matter.Conclusion Taurine can inhibit inflammatory reactions from damage induced by cerebral ischemia reperfusion and exert its neuroprotective effect on brain tissue.