Effects of As2S2 and indirubin on apoptosis and related proteins of apoptosis in MUTZ-1 cell of myelodysplastic syndrome
YANG Xiupeng
ZHOU Qingbing
MA Rou
WANG Hongzhi
HU Xiaomei
XU Yonggang
Abstract：Objective To investigate the effects of As2S2 and indirubin of Qinghuangsan main ingredient on the cell cycle,apoptosis and apoptosis-related proteins Bcl-2,Bax and Caspase-3 in myelodysplastic syndromes (MDS) cell line MUTZ-1.Methods Experiment from June 2015 to June 2016 in China Academy of Chinese Medicine Xiyuan Hospital onset laboratories.With MDS-RAEB cell lines MUTZ-1 as the research object,the experiment including 4 groups:control group,group As2S2,indirubin group and combined group (As2S2 and indirubin joint application).To the As2S2,indirubin and two joint role respectively MUTZ-1 48 h and 72 h,cells by flow cytometry to detect the cell cycle,with Annexin V-FITC/PI method to detect cell apoptosis,with flow cytometry to detect apoptosis related proteins the Bcl-2,Bax and Caspase 3-expression.Results As2S2 and ndirubin for MUTZ-1 cells have obvious inhibitory effect.Compared with the control group,the As2S2 group and combined group at 48 h S phase and G2 / M phase cells to reduce,the proportion of G0/1 cell percentage increase (P ＜ 0.05);The percentage of S phase cells during 72 h,1 phase G0 / cells percentage increase (P ＜ 0.01),the proportion of G2 / M phase cells has no obvious changes (P =0.842).Group compared with control group,indirubin cells in different time points in each cell cycle has no obvious changes (P ＞ 0.05).And As2S2 compared two groups and indirubin group,a joint group of at 48 h S period and G2 / M phase cells proportion reduce G0/1 phase is increased (P ＜ 0.05) 72 h G0/1 phase proportion increases (P ＜0.01).Compared with control group,the As2S2 groups and indirubin early apoptosis cells increased significantly (P ＜ 0.05).With the As2S2 and indirubin group,united group to promote early apoptosis effect also enhanced (P ＜ 0.05).Compared with control group,indirubin group and combined group at 48 h cells express Caspase-3 increase (P ＜0.01),the As2S2 group at 72 h cells expressing the Bcl-2 cut (P ＜ 0.01),increased Bax (P ＜ 0.05).Compared with the As2S2 and indirubin group,combined group of MUTZ-1 cell the Bcl-2,Bax and Caspase-3 expression had no effect (P ＞ 0.05).Conclusion Qinghuangsan as main ingredient As2S2 and indirubin cells by promoting MDS MUTZ-1 apoptosis,influence of apoptosis related proteins play a role in the treatment of MDS,and use the As2S2 inhibits MUTZ-1 cell proliferation,and indirubin joint application can enhance the regulation of cell cycle and promote the role of cell apoptosis.
Keywords：As2S2NdirubinMyelodysplastic syndromeMUTZ-1 cellApoptosis
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 1132-1136 )