Correlation of Qiliqiangxin capsule on myocardial fibrosis and expression of TGF-β1/Smad3 signaling in rats with chronic heart failure

ZHANG Li; XU Xu; WAN Yong; GE Ying; WANG Daqing; ZHOU Jianzhong

doi:10.3969/j.issn.1671-6450.2017.06.019

Abstract：Objective To explore the mechanism of chronic heart failure (CHF) by observing the effects of Qiliqiangxin capsule on myocardial fibrosis and expression of CTGF of rats with CHF.Methods The experiment was carried out in the animal experimental center of First Affiliated Hospital of Chongqing Medical University from April 2012 to October 2012.Eight weeks after chronic heart failure,the rats were randomly divided into the sham-operation group,the model group,the high-dose of Qiliqiangxin capsule group,the middle-dose of Qiliqiangxin capsule group,the low-dose of Qiliqiangxin capsule group and the enalapril group.After 4 weeks treatment,plasma brain natriuretic peptide (BNP) and left ventricular weight index (LVWI) were detected,Picric-Sirius Red Polarimetry was used to detect the collagen type of left ventricular interstitial tissue,collagen volume fraction (CVF) were measured,and expression of TGF-β1/Smad3 in myocardium were evaluated by immunohistochemical staining and western blot analysis.Results Compared with the sham-operated group,BNP, LVMI,I/III CVF,TGF-B1/Smad3 expression of model control group increased significantly (t=9.75,2.04,9.59,8.57,9.23,P=0.001,P=0.001,P=0.001,P=0.008,P=0.008);Compared with the model group,LVMI,Type I CVF,Type III CVF, TGF-β1/Smad3 expression of Qiliqiangxin capsule groups decreased (P<0.05,P<0.01).Compared with the enalapril group,high-dose and middle-dose of Qiliqiangxin capsule groups had no obvious changes of all the indices (P>0.05).Conclusion Qiliqiangxin capsule obviously attenuates myocardial remodeling and myocardial fibrosis of rats with chronic heart failure,improves cardiac function,which is dependent on its effect on expressions of TGF-β1/Smad3.