The effect of HMGB-1 in serum and bronchial lavage fluids in patients of lung cancer

CHI Yuhua; YANG Zhaoxia; ZHANG Xiuyan; DU Lifa; WANG Shangui; DING Zhaojun

doi:10.3969/j.issn.1671-6450.2017.05.012

Abstract：Objective To evaluate the correlation value between the level of serum HMGB-1 and bronchial lavage fluids and cancer metastasis in patients of lung cancer.Methods Ninety four lung cancer patients and eighty pneumonia benign disease patients were enroll in this study. The contents of serum HMGB-1 and bronchial lavage fluids were measured and recorded. Correlation analysis was used to estimate the relation between serum HMGB-1 and tumor stage. Results The level of serum HMGB-1 of and bronchial lavage fluids in lung cancer group were significantly higher than those in control group[serum:(5.4±0.4)ng/ ml vs. (1.4±0.3)ng/ ml,t=2.055,P=0.024; BLF:(12.4±2.5)ng/ ml vs. (2.4±0.5)ng/ml,t=2.831,P=0.004].The contents of serum HMGB-1 were higher than those of bronchial lavage fluids in lung cancer patients[(5.4±0.4)ng/ml vs. (12.4±2.5)ng/ml,t=2.327,P=0.013].Compared with Stage III-IV patients, the HMGB-1 levels of both serum and bronchial lavage fluids in I-II patients were increased[serum:(4.2±0.3)ng/ ml vs. (6.9±0.8)ng/ml,t=2.430,P=0.010;BLF:(9.4±1.9)ng/ml vs. (14.7±3.5)ng/ml,t=2.565,P=0.008].The lung cancer patients with lymphatic metastasis had higher levels of serum HMGB-1 and bronchial lavage fluids compared with those without lymphatic metastasis. ROC analysis showed that the AUC for lung cancer diagnosis of serum and bronchial lavage fluids of HMGB-1 were 0.722 and 0.828 respectively. Correlation analysis showed that both serum and bronchial lavage fluids of HMGB-1 were positively related with lung cancer. Conclusion The levels of serum and bronchial lavage fluids HMGB-1 are elevated in lung cancer patients and used for early detection and stage prognosis.