Effect of electrical stimulation to cerebellar fastigial nucleus on the protein and gene expressions of nuclear factor kappa B/p65 and TNF-α after focal cerebral ischemia reperfusion in rat brain
GONG Jiaming
JIANG Yingzi
ZHAO Xue
HU Derong
QU Xiang
XIE Xuemei
Abstract：Objective To investigate the mechanism of neuroprotection of cerebellar fastigial nucleus electrical stimulation (FNS) by means of observation of the effect of FNS on the protein and gene expressions of NF-κB/p65 and TNF-α after focal cerebral ischemia reperfusion in rat brain.Methods In the Medical University of Chongqing Laboratory of Neurology,from September 2014 to September 2015,sixty Wistar rats were randomly divided into normal group (NC),cerebral ischemia reperfusion group (I/R) and cerebral ischemia reperfusion group with a FNS treatment (FNS),models of focal cerebral ischemia reperfusion injury were established by middle cerebral artery occlusion (MCAO).Expression of NF-κB/p65 and TNF-α was measured by RT-PCR and immunohistochemistry.The absorbance value was measured by imaging analysis.Results Ischemia reperfusion group compared with the normal group,the expressionof NF-κB/p65 and TNF-α increased (q =0.032,q =0.020,P ＜ 0.05),electrical stimulation of fastigial nuclear intervention,NF-κB/p65 and TNF-α expression were significantly reduced,and ischemia reperfusion group were significantly different (q =0.182,q =0.013,P ＜ 0.05).Compared with the NC group,the expression of NF kappa B/p65 mRNA and TNF-α mRNA was significantly higher in group I/R (P ＜ 0.05).The expression of NF kappa mRNA and TNF in FNS group was significantly decreased (P ＜ 0.05) compared with I/ R group.Conclusion Fastigial nucleus stimulation inhibits NF-κB/p65 activation and TNF-α transcription,NF-κB/p65 activation and expression was inhibited due to the reduction and the expression of TNF-α may be its one of the mechanisms of central nervous protective effects.
Keywords：Cerebellar fastigial nucleus electrical stimulationCerebral ischemia reperfusionNF-κB/p65TNF-αRats
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 284-286,292,封3 )