The impact of PGZ joint 5-AzaC intervention on pancreatic βcell proliferation, apoptosis and function
LI Li
LI Song
SHI Xiaojuan
WANG Shunge
WANG Xue
CHEN Min
Abstract：Objective To investigate pioglitazone ( PGZ) combined with 5 azacytidine (5-AzaC) intervention on in-sulin the proliferation , apoptosis ofβcell proliferation and insulin βcell insulin secretion .Methods The experiment was car-ried out in Department of Endocrinology , Second People's Hospital of Henan province from June 2015 to June 2016 in Ping-dingshan.The rat insulinoma cell line (RIN-m5f) in vitro and cultured in vitro, the cultured cells were divided into 5 groups:control group ( without any reagent ) , model group ( IL-1β2 ng/ml+IFN-γ100 U/ml) ,pioglitazone group ( IL-1β2 ng/ml +IFN-γ100 U/ml+PGZ 15 μmol/L),5-AzaC group ( IL-1β2 ng/ml +IFN-γ100 U/ml +5-AzaC 1.5 μmol/L) and PGZ+5-AzaC group (IL-1β2 ng/ml+IFN-γ100 U/ml +PGZ 15 μmol/L +5-AzaC 1.5 μmol/L).The inverted micro-scope was used to observe the change of morphology of RIN-m5f cells,RIN-m5f-determination of cell apoptosis by flow cytome-try and the detection of B cell lymphoma by Western blot 2 (Bcl-2), polysaccharides (PGZRP) expression was detected by ELISA, glucose stimulated insulin secretion.Results The apoptotic rate of RIN-m5f cells in PGZ group, 5-AzaC group and PGZ +5-AzaC group was lower than that in the model group after 24,48,72 h( q PGZ group=12.122,8.452,8.252, P <0.05;qt5-AzaC group =12.252、8.563、7.529, P <0.05;q PGZ+5-AzaC group =8.563、7.452、8.963, P <0.05).The apoptosis rate of PGZ+5-AzaC group was significantly lower than that of PGZ group and 5-AzaC group ( q PGZ group =7.022、6.986、8.523, P <0.05;q 5-AzaC group =8.963、9.123、10.523, P <0.05),but the difference was not statistically significant ( P >0.05).The apoptosis rate in PGZ +group was significantly lower than that in PGZ +group ( P >0.05).The expression of Bcl-2 and PGZRP was decreased in model group >PGZ group>5-AzaC group>PGZ +5-AzaC group.PGZ +5-AzaC group glucose stimulation better than PGZ group and 5-AzaC group ( P <0.05).The insulin secretion ability of the 5-AzaC group was sig-nificantly higher than that of the PGZ group , 5-AzaC group ( q =8.252, q =7.896, P <0.05).Conclusion The PGZ combined with 5-AzaC can effectively inhibit the apoptosis of insulinoma induced by high glucose , and restore the ability of in-sulin secreting tumor.The mechanism may be related to the down regulation of the expression of Bcl-2 and PGZRP.
Keywords：Pioglitazone5 azacytidineRIN-m5f cellsB cell lymphoma 2Polysaccharide polymeraseRats
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 182-185,192 )