Effect of KuijielingⅣon NF-κB, TNF-αin colon tissues of ulcerative colitis rats

ZHENG Jixiang; CHENG Limin; GAO Longying

doi:10.3969/j.issn.1671-6450.2017.02.018

Abstract：Objective To observe the effect of KuijielingⅣretention enema on nuclear factor kappa B ( NF-κB) ex-pression and tumor necrosis factor alpha ( TNF-α) content in colon tissues of ulcerative colitis ( UC) rats induced by trinitro-benzene sulfonic acid (TNBS), discussing the possible mechanism for the treatment of UC .Methods The experiments were operated in June 2015 to August 2015 , in the life science and engineering laboratory of Harbin industrial university college . Choosing 12 in 138 SD rats randomly as blank control group (BC), the rest of rats were produced UC rat model induced by the method of TNBS enema and were randomly divided into model control group ( MC) , KuijielingⅣhigh dose group ( HD) , medium dose group ( MD) , low dose group ( LD) , positive control group ( PC) .HD, MD, LD group were treated respective-ly with high, medium and low dose of KuijielingⅣretention enema , PC group was treated by the mesalazine retention enema , BC and MC group were given for the same volume of distilled water .Using immunohistochemical method to detect the NF-κB protein expression in colonic mucosa , ELISA method for determining the content of TNF-αand colon of rats damage rating number etc.several aspects to observe the effect of KuijielingⅣretention enema on UC rats colon tissues induced by TNBS . Results (1) colorectal colon damage:the colon tissue of rats BC clear structure , mucosa, intestinal gland rich , closely ar-ranged;MC group made in about 1 weeks after rat colon tissue injury is the most serious;colonic mucosa necrosis , ulcer for-mation, massive neutrophil infiltration, PC, glands disappear;HD, MD, LD group after administration, the rat colon damage are gradually reduced .LD, MD, HD, PC group of rats colon showed intestinal mucosal epithelial defect reduced , ulcer area significantly reduced mucosal epithelial hyperplasia around repair , partial coverage , inflammatory cells decreased , granulation tissue hyperplasia significantly .Compared with the MC group , the colon injury in HD and PC group was significantly reduced . (2) NF kappa B protein expression: BC group of rat colon tissue only a small number of cells within the cytoplasm visible yellow particles , MC group cytoplasm nucleus are visible brown yellow and brown particles .With the passage of time , the ex-pression of NF kappa B in colon tissue of rats in each group was decreased .Compared with MC group , the expression of NF kappa B in MD, HD and PC group were significantly lower ( P <0.05), and there was no significant difference between LD group and MC group ( P >0.05).(3) TNF-αcontent:TNF-αcontent of colon tissue in each group were higher than that of group BC, compared with the MC group, 7 d after modeling, LD, MD, HD, PC group of rat colon tissue TNF-αis increased, but started from 14 d, LD, MD, HD, PC the group had decreased , PC, HD group and MC group were significantly different , and LD, MD, HD group showed an obvious dose effect relationship.Co nclusion KuijielingⅣ retention enema has certain protective effect on colon of UC rats induced by TNBS and its mechanism may be related to inhibit the expression of the NF -κB and reduce the inflammatory factor content of TNF-α, consequently reducing the inflammatory stimulation for colon tissues of rats by pro-inflammatory factor , achieving the protection effect on colon tissues of UC rats .