Experimental study of the apomorphine, sildenafil and female mice induced in the impact of diabetic rats erectile function

MA Kaiwei; SONG Guohong; ZHANG Qiumei; GAO Xuan

doi:10.3969/j.issn.1671-6450.2017.01.018

Abstract：Objective To observe and compare the induced effect on erectile function in diabetic rats and female rats of apomorphine , sildenafil .Methods Thirty male Wistar rats , 6 rats in normal control group , the remaining 24 in the intra peritoneal injection of streptozotocin (STZ) induced diabetic model, successfully induced diabetic rats and 22 rats survived, according to blood glucose will be only 18 of them were divided into 3 groups, 6 rats in each group:apomorphine group (80μg/kg apomorphine was injected intraperitoneal ) , sildenafil group ( 10 mg/kg sildenafil was gavaged ) and model group , all male rats were induced by female rats ,and recorded the changes in the number of times of capture ride in 60 min of 3 groups. Results Drugs by STZ intraperitoneal injection of 25 mg/kg +surface area method , the method of successful establish the model rate was 91.7%(22/24), mortality rate 8.3%(2/24), so using STZ drugs 25 mg/kg +surface area method of in-traperitoneal injection is a safe , reliable, high successful establish the model rate method .Sildenafil group, apomorphine group and blank control group 3 group blood glucose had no statistically significant difference ( F =0.289, P >0.05);Silde-nafil, apomorphine group compared with normal control group , the number of capture , riding a number of differences ( F =32.457,40.483, P <0.01), but the blank control group compared with normal control group , number of capture, ride a number difference is not obvious (P> 0.01).Conclusion Diabetic rats by apomorphine and sildenafil after drug stimula-tion indicate more of the drugs , cannot reflect the real state of erectile function in diabetic rats , the erectile function of diabetic rats induced by state to determine the use of female rats reflect the erectile function in diabetic rats , so as to provide the basis for drug treatment.