CGRP and SP expression of colorectal mucosa in patients with irritable bowel syndrome and its correlation with clini-cal symptoms

FANG Liang; ZHANG Fanyong; WU Jixiong

doi:10.3969/j.issn.1671-6450.2017.01.016

Abstract：Objective To investigate the expression of calcitonin gene-related peptide(CGRP) and substance P(SP) in colorectal mucosa in patients with different types of IBS , and the correlation between SP and CGRP and clinical symptoms of IBS .Methods 63 cases of IBS in our hospital from 2012 to 2014 in the Department of digestive medicine were selected , Constipation type IBS 24 cases, diarrhea type IBS 21 cases, mixed type IBS 18 cases,10 cases of colon polyps were selected as the control group .Immunohistochemistry was used to detect the expression of CGRP and SP in colorectal mucosa .Results Granular SP staining was found in the cell membrane , abundant in the cytoplasm of gland cells .The gland around Brown gran-ular glands SP , individual cell membrane Brown granular SP .CGRP immunoreactive nerve fibers were brownish yellow granu-lar, round, oval or irregular shape , aggregation or scattered in the mucosal glands , CGRP expression of IBS group was signifi-cantly increased .Diarrhea predominant IBS patients CGRP expression was significantly higher than that of the constipation predominant IBS, mixed type of irritable bowel syndrome (IBS) and control group (P <0.01) in the ileocecal valve and sig-moid colon;Diarrhea predominant IBS patients SP expression was significantly higher than that of the constipation predominant IBS, mixed type of irritable bowel syndrome ( IBS) and control group ( F CGRP =21.64,3.41, F SP =5.26,5.41,all P <0.01) in the ileocecal valve and sigmoid colon; CGRP, SP high expression group in defecation difficulty , abdominal pain, stool character were statistically significant difference from low expression group , moderate expression group ( F CGRP =3.85, 31.76,13.929, F SP =3.450,28.070,9.287, P <0.05).CGRP were positively correlated with abdominal pain ,bowel move-ment traits( r =0.999, r =0.999, P <0.05), and negatively correlated with defecation difficulties ( r =-0.993, P <0.05);SP were positively correlated with abdominal pain ,defecate traits( r =0.997, r =0.984, P <0.05),and negatively correlated with difficult defecation ( r =0.940, P <0.05), stool frequency( r =-0.997, P <0.05).Conclusion The expression of CGRP and SP was significantly increased in patients with diarrhea type IBS , SP and CGRP can affect the defeca-tion difficulties , abdominal pain and stool characteristics and other symptoms .