Gene chip analysis of differentially expressed genes in AS model of ApoE mice by blood activating drugs and blood breaking drugs

ZHOU Lan; LI Yang; WANG Dian; LU Qing; WANG Min; CHEN Xu; LUO Yaoyue; XIE Haibo

doi:10.3969/j.issn.1671-6450.2017.01.005

Abstract：Objective Using gene chip technology on load apolipoprotein E ( ApoE) gene in the knockout mice ath-erosclerosis model in thoracic aorta in differentially expressed genes , the expression and discuss the mechanism of different do-ses of drugs for promoting blood circulation ( Ligusticum chuanxiong and Angelica sinensis ) , and blood breaking drugs ( tri, Ezhu) screening.Methods The C57BL/6 mice were used as blank control group (blank group).64 ApoE gene knock mice with 5 weeks according to the random number table method , were divided into model group ( model group ) , atorvastatin atorv-astatin calcium group ( atorvastatin group ) , low Huoxue treatment group ( low activity group ) , low broken blood medicine group ( low carbon group ) , high blood group ( high activity group ) , high rupture blood drug group ( high rupture group ) , dai-ly were given ordinary feed to duplicate the model of atherosclerosis .After successful modeling , the blank group and the model group were administrated by volume of distilled water , other groups mice were orally administered , 1 times a day, for 8 weeks, in the treatment of animals , with differences in gene chip technology to detect the mouse aortic tissue expression of genes .Re-sults The blank group compared with model group , there were 154 differentially expressed genes , including 90 genes up-reg-ulated and 64 genes were down regulated;the model group compared with atorvastatin group , there were 96 differentially ex-pressed genes , including 17 genes up-regulated and 79 genes were down regulated;the model group compared with the low ac-tivity group, the expression of 214 genes the difference , of which 95 genes were upregulated and 119 genes were down regula-ted;the model group compared with the low carbon group , there were 134 differentially expressed genes , including 87 genes up-regulated and 47 genes down regulated;high activity group compared with the low activity group , there were 236 differenti-ally expressed genes , including 169 genes, 67 gene expression; high breaking group compared with the low carbon group , there were 184 differentially expressed genes , including 88 genes up-regulated and 96 genes down regulated .AS related genes are:compared with model group , the expression of Cxcl16, Alox12, VcamI and Cxcr4 in blood circulation drug group and drug group was down regulated , and the expression of Alox5ap, Cxcl12, LCAT and Ccr5 were up regulated.Conclusion Blood activating drugs ( angelica sinensis and ligusticum wallichii ) , and blood breaking drugs ( rhizoma sparganii and curcuma zedoary) show anti atherosclerotic effect and its mechanism may be related to multiple genes occur expression level changes .