The mechanism of preconditioning on renal ischemia reperfusion injury in rats and the effects of dexmedetomidine under high glucose condition
GAO Wenwei
WANG Huaxin
ZHAO Bo
WU Yang
JIANG Meng
Abstract：Objective To investigate the mechanism of preconditioning on renal ischemia reperfusion injury in rats and the effects of dexmedetomidine under high glucose condition .Methods From October 2014 to June 2015 , in the Central Laboratory of Renmin Hospital of Wuhan University , 36 adult male SD rats were randomly divided into:sham operation group (NG-sham), ischemia and reperfusion group (NG-I/R) and dexmedetomidine preset treatment group (NG-Dex), high glu-cose sham operation group ( HG-Sham) , sugar ischemia again perfusion group ( HG-I/R) , high glucose dexmedetomidine pre-set treatment group (HG-Dex) with 6 rats in each group.The renal ischemia reperfusion model was made in all groups except the sham operation group , Dex pretreatment group was injected Dex 50μg/kg 30 min before ischemia .Cell apoptosis was de-termined by in situ terminal transferase labeling (TUNEL) method.Blot Western method was used to detect Bax , Bcl-2, pro-tein kinase B (AKT) and phosphorylated AKT (p-AKT).Results Compared with NG Sham group, NG-I/R group can be seen in tubular damage obviously , including renal tubular expansion , renal tubular epithelial cell swelling , nucleus proprius and inflammatory renal tubular necrosis , apoptosis index, Bax, p-AKT were increased, Bcl 2 was decreased ( F =6.667, F =469.889, F =1.200, F =0.791, F =0.499, P <0.05);compared with NG I/R group, NG-Dex group structure was normal, visible to the normal structure of renal tubule and renal tubular cells , apoptosis index and Bax decreased , and p-AKT, Bcl-2 increased ( F =59.333, F =27.667, F =0.028, F =0.034, F =0.024, P <0.05); HG-I/R group, HG-Dex cell damage the degree of apoptosis index , Bax, p-AKT were higher than that of NG I/R group and NG-Dex group, the Bcl 2 lower than that of the corresponding group ( F =66.000, F =497.556, F =1.227, F =0.824, F =0.523, P <0.05); HG-I/R group, HG group's p-AKT Dex apoptosis index had no significant difference ( P >0.05).Conclusion Dex has a protective effect on normal renal ischemia-reperfusion injury , but this effect is impaired in a high glucose environment , which may be related to the expression of p-AKT, regulation of Bcl-2 and Bax, and increased renal I/R damage.
Keywords：High glucoseDexmedetomidinePretreatmentKidney ischemia reperfusionRats
Publication Date：2016-01-01
Pages：4( 955-958 )