Construction of human BMP-7 adenovirus and its expression after transfection of canine bone marrow stromal stem cells
PU Zhichao
XIE Weiyong
ZHANG Xingshi
HE Canjie
HUANG Furong
RUAN Xiaoping
Abstract：Objective To explore the construction method of the recombinant adenovirus carrying human bone mor -phogenetic protein-7(BMP-7) and its expression after transfection of canine bone marrow stromal stem cells .Methods To construct vectors that carrying human bone morphogenetic protein -7 adenovirus in vitro using the adenoviral expression system and transfect into the human embryonic kidney 293 cells for homologous recombination, using recombinant adenovirus carrying human BMP-7 gene to transfect canine bone marrow mesenchymal stem cells , then to detect BMP-7 gene expression by the RT-PCR method.Results Restriction analysis proved that the BMP -7 transfer plasmid shuttle vector pTrack -BMP-7 and BMP-7 recombinant adenovirus genome had been obtained and a recombinant adenovirus was packaged , the virus titer was 3.6 ×1012 VP/ml.RT-PCR proved that BMP-7 gene was expressed in canine bone marrow mesenchymal stem cells which were transfected by recombinant adenovirus , the transfection efficiency was about 98%.Conclusion The successful construction of BMP-7 recombinant adenovirus and the successful transfection of canine bone marrow mesenchymal stem cells and their high expressions provide an experimental basis for the gene treatment of bone defect and nonunion .
Keywords：Bone morphogenetic protein-7AdenovirusBone marrow stromal cellsDog
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 736-741 )