Efects and mechanisms of dexmedetomidine preconditioning on ischemia-reperfusion arrhythmia in rats
LI Xia
LIU Fen
DONG Hang
JIANG Qun
XIONG Haixia
Abstract：Objective To investigate effects and mechanisms of dexmedetomidine preconditioning on ischemia-reper-fusion arrhythmia in rats.Methods From March to June 2015 in Basic Mesical College of Wuhan University,30 rats were di-vided into sham operation group ( SO group) , ischemia-reperfusion group ( I/R group) and dexmedetomidine preconditioning group ( DEX group) ,10 rats each group.Firstly, the coronary artery was ligated for 30 min to cause myocardial ischemia, and then the ligature was loosened for 120 min to give the myocardium reperfusion.Lines were given but not ligated in SO group, 100 μg/kg Dexmedetomidine was given intra-peritoneal at 2h before ischemia in DEX group, and the same volume physiologi-cal saline was given in I/R group.The type and the scores of arrhythmia were monitored and recorded in each group.The mR-NA level of Cav1.2 and NCX were detected by the real-time PCR.Results There was no significant changes of cardiac rhythm in SO group, and the score of arrhythmia was 0.The incidence of arrhythmia were lower in DEX group than in I/R group ( P <0.05), and the scores of arrhythmia within 30 min of ischemia and 120 min of reperfusion in DEX group were both lower than in I/R group ( P <0.05).Compared with SO group, the mRNA levels of Cav1.2 and NCX increased in both I/R group and DEX group ( P <0.05), and which were significantly lower in DEX group than in I/R group.Conclusion Dexmedetomidine preconditioning can reduce the incidence of arrhythmia, which is related to the mechanism that dexmedetomi-dine reduces Cav1.2 and NCX mRNA levels to inhibit calcium overload.
Keywords：DexmedetomidinePreconditioningIschemia-reperfusion injuryArrhythmiaRats
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 514-517 )