The clinical significance of quantitative measuring urinary glycosaminoglycans by Blyscan method in mucopolysaccha-ridosis patients
DUAN Jinyan
YUAN Xiaozhou
MENG Yan
WANG Chengbing
Abstract：Objective To study the efficiency of the Blyscan method for measuring urinary glycosaminoglycans ( GAGs) in mucopolysaccharidosis ( MPS) patients and its clinical significance.Methods The intra-day and inter-day preci-sions , temporal stability , average recovery were detected by Blyscan method in the study.The urinary GAGs of 25 cases of MPS patients ( MPS group) diagnosed by pediatric department and 20 health cases ( normal control group) from October 2013 to June 2015 were detected by Blyscan method.Results The CV values of the intra-day precisions for detecting low-value and high-value standard solution by Blyscan method were 0.96%and 1.32%,and the CV values of inter-day precisions of them were 2.33%and 1.85%,respectively.And what’s more,the recoveries of 10 and 20 mg/L detected by Blyscan method and DMB-Tris method were (97.04 ±3.61)%,(100.32 ±5.62)% and (95.01 ±1.62)%,(103.9 ±2.81)%, respectively, without significantly difference ( P >0.05).However, the stability of Blyscan method was much more stable than DMB-Tris method ( P <0.05) .Compared the value of urinary GAG/Cr between the MPS patients and control, the former was significant higher than the latter [(65.36 ±21.96)mg/mmol vs.(12.32 ±1.75)mg/mmol,( P <0.05)].Conclusion It proved that the Blyscan method ,which is suitable for the clinical quantitative detection of GAGs content, was a reliable, stable and simple GAGs quantitative assay method .
Keywords：MucopolysaccharidosisGlycosaminoglycansBlyscan methodDMB-Tris method
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 472-475 )