Research on correlation between 8-hydroxy deoxyguanosine level and type 2 diabetes kidney disease and risk factors analysis
WU Mengshui
LI Zhenzhen
NING Cuili
LIU Kuanzhi
Abstract：Objective To observe the serum 8-hydroxy guanin(8-OHdG) level in different stage of type 2 diabetic kidney disease( DKD) patients and explore the correlation between 8-OHdG and DKD.Methods Eighty normal subjects ( NC group) and 228 patients with type 2 diabetes (T2DM) were enrolled in this study.The T2DM patients were all hospitalized from department of endocrinology, the third hospital of Hebei medical university since 2014 May to 2015 Oct.The T2DM pa-tients were further divided into three subgroups:simple type 2 diabetes mellitus ( SDM) subgroup ( n =80) , micro albumi-nuria ( mic-DKD) subgroup ( n =70) and macro albuminuria ( mac-DKD) subgroup ( n =78) according to the urinary al-bumin creatinine ratio.The level of serum 8-OHdG level in five groups was determined by enzyme linked immunosorbent assay (ELISA).The correlation between 8-OHdG and FPG, HbA1c, TC, TG, LDL-C, HDL-C, SCr, BUN,UA and urinary A/Cr were also analyzed.Logistic regression model was used to analyze the risk factors of DKD.Results (1)The serum 8-OHdG level in T2DM group was(7.20 ±2.37) ng/ml, which was higher than the NC group (3.94 ±0.26) ng/ml( t =20.418, P <0.01);Compared between subgroups:The serum 8-OHdG level in mac-DKD subgroup was(9.68 ±1.17)ng/ml, which was higher than the mic-DKD subgroup(7.34 ±1.32)ng/ml( t =11.365, P <0.01) and SDM subgroup(4.65 ±0.74)ng/ml( t =32.206, P <0.01).(2)Correlation analysis:The serum 8-OHdG level was positively correlated with urinary A/Cr (r =0.818), TG ( r =0.745), TC ( r =0.669), FPG ( r =0.662), HbA1c( r =0.661), LDL-C ( r =0.639), SCr ( r =0.339) and UA ( r =0.242) (all P <0.01), negatively correlated with HDL-C ( r =-0.628, P <0.01).(3)Lo-gistic regression analysis showed that 8-OHdG (OR=11.133,95%CI 3.359-36.895, P =0.000),TG (OR=65.573,95%CI 4.400-977.265, P =0.030),HbA1c(OR=1.673,95%CI 1.019-2.744, P =0.042), duration (OR=1.567,95%CI 1.182-2.077, P =0.002) and age (OR=1.106,95%CI 1.006-1.216, P =0.038) were independent risk factors for the occurrence of DKD.Conclusion The level of serum 8-OHdG can reflect the severity of DKD, oxidative stress is associated with the occurrence and progression of DKD.
Keywords：8-hydroxy guaninOxidative stressDiabetic nephropathyRisk factors
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 460-464 )