Expression level and clinical significance of SIRT1 in patients with pancreatic cancer

WU Ruoshi; LU Haishan; WEI Huamei; LUO Chunying

doi:10.3969/j.issn.1671-6450.2016.04.015

Abstract：Objective To detect silent information regulator1( SIRT1) mRNA and protein expression levels of pancre-atic cancer tissues, investigate its relativity with the clinicopathology in pancreatic cancer and evaluate its clinical significance in the auxiliary diagnosis and prognosis of pancreatic cancer.Methods From Febrary 2010 to June 2012, SIRT1 mRNA ex-pression was detected by real-time PCR in 31 fresh pancreas cancer tissues, which were collected in Affiliated Hospital of You-Jiang Medical College, meanwhile 21 pancreatitis tissues were paired as control.And SIRT1 protein expression level was also measured by immunochemistry in 46 cases of pancreas cancer tissues and 25 paired cases of acute or chronic pancreatitis tis-sues.The relationship between SIRT1 expression and prognosis of pancreatic cancer were analyzed.Combined with clinico-pathological data and follow-up data,statistical analysis was performed by using SPSS 19.0 statistical software.Results The positive rates of expression levels of SIRT1 mRNA and protein in the patients with pancreas cancer was significant higher than that in the pancreatitis controls ( P <0.05).The positive rate of pancreatitis paraffin block of SIRT1 protein was 44.0%(11/25), which was lower than pancreatic cancer paraffin block of 84.8% (39/46), the differences was statistically significant (χ2 =12.934, P <0.05).In pancreatic cancer tissues, SIRT1 protein expressions in different tumor stage, tumor size, lymph node metastasis and CA199 levels all exist difference.The survival curve of SIRT1 negative patients was better than that of SIRT1 positive patients;the difference was statistically significant ( P <0.05).Cox regression model analysis showed that the tumor size and clinical TNM staging were independent prognostic factors for 3-5 years in pancreas cancer.Conclusion The SIRT1 gene and protein is highly expressed in the pancreas cancer,and there are strong correlations to pathological stage, tumor size, lymphoid metastasis and CA199 level.SIRT1 expression is a prognostic factor in patients with pancreatic cancer, and its expression level has certain significance for the diagnosis and prognosis of pancreas cancer.