Correlation between the polymorphism of nirt ic oxide synthase gene and the incidence of coronary heart disease in Uygur
SHAN Xuefeng
SUN Qingchao
JIN Xiaochuang
MA Songfeng
Abstract：Objective To analyze the correlation between the 3 polymorphisms of endothelial nitric oxide synthase ( eNOS) gene G894T and 4a4b and the incidence of coronary atherosclerotic heart disease ( CHD) in Xinjiang Uygur popula-tion.Methods Choose from January 2012 to December 2014, 136 cases of patients with CHDin the First Affiliated Hospital of Xinjiang Medical University, Department of coronary heart disease enrolled as the study group, while selecting the same pe-riod of 120 healthy people for physical examination as control group.Sampling the elbow vein blood and by the polymerase chain reaction restriction fragment length polymorphism ( PCR-RFLP) and PCR analysis of single nucleotide polymorphisms G894T polymorphisms, and a variable series repeats (VNTR) loci 4a4b, testing each of the genotype and allele frequencies and compared between the two groups in each genotype and allele frequency differences.Results TT, GT genotype of eNOS gene G894T in study group were significantly higher than healthy control group ( OR=2.16, 95% CI 1.12 -3.68, P <0.05), the frequency of T allele was higher than the control group (OR=1.96, 95CI, 1.09-3.76, P <0.05).The 4a4b allele frequency of 4A was significantly higher than that in healthy control group ( OR=2.18, 95CI, 1.31 -3.58, P <0.05).Conclusion The genotype and allele frequency distribution of G894T gene eNOS and 4a4b polymorphism was related to the incidence of CHD in Xinjiang Uygur population, the allele T and allele 4A may be the susceptibility gene of CHD pa-tients in Xinjiang Uygur Autonomous Region.
Keywords：Coronary heart diseaseNitric oxide synthasePolymorphismUygur nationality
Publication Date：2016-01-01
Pages：6( 335-339,343 )