Experimental study of skeletal muscle ischemia reperfusion induced lung injury in rats
ZHANG Yanrong
ZHU Tia-nyi
WU Zhifeng
BI Wei
Abstract：Objective To observe the changes of lung injury caused by different ischemia time of skeletal muscle . Methods Twenty-four male Wistar rats were randomly assigned for four groups , group I as the control group , only general anesthesia , did not block the hind limb blood flow;group II for blocking hind limb blood flow for 2 h and reperfusion for 6 h;group III for blocking hind limb blood flow 4 h and reperfusion 6 h;group IV for ischemia 8h and 6 h of reperfusion;detected rat’s serum lactate dehydrogenase (LDH) and malondialdehyde (MDA) content, muscle tissue and lung tissue pathological changes.Results (1) LDH levels:after the experiment , changes in group I is not obvious , group II, III and IV showed a rising trend, the difference were significant compared with group I ( P <0.05), and the group IV >group III >group II (P <0.05).(2) The level of MDA:after the experiment, group I and group II changes were not obvious , and group III and IV showed a rising trend, and group IV >group III >group II and I ( P <0.05).(3) The microscopic findings of the muscle tissue were increased with the increase of ischemia time , and the damage of the IV group was severe .But lung tissue injury only found in group III and IV , and gradually increased the level of blocking time .Conclusion Rat skeletal muscle is-chemia and after reperfusion tissue damage in accordance with the prolongation of ischemia time and aggravating , ischemia for 4 h and reperfusion 6 h can occurred with lung injury .
Keywords：Skeletal muscleIschemia reperfusion injuryLung injuryLactate dehydrogenaseMalondialdehydeRats
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 1277-1279 )