The effect of microRNA-17 expression on oxidative stress in endothelial cells
YANG Shuo
CHEN Jing
HU Qi
JIANG Hong
Abstract：Objective To investigate the effect of microRNA-17 ( miR-17 ) expression on endothelial cell oxidative stress.Methods Cultured original generation umbilical vein endothelial cells ( HUVEC) , they were transfected to negative control virus [Ad(N) group], up-regulation of miR-17 adenovirus (Ad-Pre-miR-17) and down-regulation of miR-17 adenovi-rus ( Ad-Antagomir-17 group) , the release of endogenous nitric oxide ( NO) is detected by the silver nitrate method, the con-tent of NO in DAF FM probe for the detection of cell, and used DHE probe for the detection of cell super oxide ( ROS) con-tent and analyzed.Results Compared to Ad ( N) group, the content of NO in Ad-Antagomir-17 cell group increased ( P <0.05), intracellular ROS content decreased ( P <0.05); and reduce the content of NO in Ad-pre-miR-17 cells ( P <0.05), intracellular ROS increased ( P <0.05).Conclusion Reduce miR-17 can improve blood supply in patients with coronary heart disease, reduce oxidative stress and help to improve vascular function.
Keywords：MicroRNA-17Endothelial cellsNitric oxideOxidative stress
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 1173-1175,1185 )