Expression levels of microRNA-124 in plasma and tissure correlated with prognosis in patients with colorectal cancer

LV Mei; HUAN Xin; LI Qianwei; WANG Yanyan

doi:10.3969/j.issn.1671-6450.2015.11.008

Abstract：Objective To analyze the correlation between plasma and tissue miR-124 expression and overall survival ( OS) of patients with colorectal cancer.Methods Collected 71 cases of patients with colorectal cancer which cannot be re-moved ( n =49) , completely resected ( n =22) , collected the plasma and tumor tissue samples.Detected tumor unresect-able tissue and plasma of miR-101, miR-124, miR-126, miR-210, miR-217’ s expression, by quantitative real-time reverse transcription polymerase chain reaction ( qRT-PCR) to analyze the correlation between miR-124 expression and the prognosis of the patients.Results qRT-PCR for detection of unresectable expression of miRNAs in plasma and tissue in patients with colorectal cancer, high expression of miR-124’s changes revealed as the most significant ( P <0.05, P <0.01), and other miRNAs did not show significant changes.(1) Correlation between plasma miR-124 and patients’ OS:in unresectable color-ectal cancer patients, plasma and tissue expression of miR-124 was positively correlated with the OS ( OR=0.451, 95% CI 0.189 -0.654, P =0.002;OR=0.295, 95%CI 0.493-13.74, P =0.037).(2) Cox proportional hazards models as-sessment:the Cox proportional hazards model evaluation revealed that the high expression of miR-124 in plasma were correla-ted with PFS ( OR=0.147, P =0.022); and high expression of miR-124 in tissue had no correlation with PFS ( OR=0.281, P =0.057), plasma and tissue low expression of miR-124 had no correlation with low mortality risk ratio (OR=2.07, P =0.163).High expression of miR-124 had no correlation with PFS (OR=1.85, P =0.725).Conclusion It demon-strated that the plasma miR-124 expressions could be used as biological markers for evaluating the prognosis of colorectal cancer.