Correlation of glucocorticoid resistance and peripheral blood lymphocyte glucocorticoid receptor mRNA expression in children with primary nephrotic syndrome
LI Yi
WANG Jian
WAN Guangping
GU Zhibing
WANG Zhuqiang
Abstract：Objective To investigate the relationship between glucocorticoid ( GC) and the expression of glucocorti-coid receptor mRNA (GR) in patients with primary nephrotic syndrome (PNS).Methods From June 2012 to December 2014 , 80 cases of newly diagnosed pediatric PNS children who treated with high dose of corticosteroid were enrolled as study group.According to the curative effect of GC , these children were divided into hormone sensitive subgroup and resistance sub-group, and 30 cases normal children of the same age were enrolled as healthy controls .Venous blood was taken at 5 ml before treatment, and the mRNA expression level of GR αand GRβin peripheral blood mononuclear cells (PBMCs) were detected by fluorescence quantitative PCR.Results 80 cases of PNS children had 15 cases of GC resistance(18.8%),65casesof GC sensitive (81.2%), compared with the healthy control group, the study group’s PBMCs in GR αmRNA and GR beta mRNA expression levels were higher , the differences were statistically significant ( P <0.05).Compared with the subgroup of GC resistance, GC sensitive subgroup ’ s PBMCs of glucocorticoid receptor beta mRNA expression was higher , and the difference was statistically significant ( P <0.05), and GR mRNA expression level’s differences had no statistical signifi-cance ( P >0.05).Using logistic regression analysis adjusted for confounding factors , study group children’s PBMCs of glu-cocorticoid receptor beta mRNA expression levels is independent risk factors of GC resistance (OR=2.17, 95% CI 1.17~9.21, P <0.05).Conclusion PNS children’ s glucocorticoid receptor beta mRNA expression level in PBMCs is an inde-pendent risk factor for GC resistance , may use as a clinical reference index for predicting GC response .
Keywords：Children primary nephrotic syndromeGlucocorticoid resistanceGlucocorticoid receptor
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 947-949,955 )