The expression and significance of autophagy gene LC3, Beclin 1 in cardiac muscle of rat model with starvation
ZHANG Jiankai
ZHANG Xiaoping
CHU Yingxin
Abstract：Objective To observe the expression of microtubule associated protein 1 (LC3) and autophagy gene Bec-lin 1 in rat cardiac myocytes, and to investigate the effect of autophagy on the myocardium of starved rats.Methods 42 healthy male SD rats were randomly divided into normal control group ( n =6) and starvation model group ( n =36).Normal control group with normal diet, starvation model group were only with free water intake, according to the number of days of the starvation, they were divided into 1d, 2d, 3d, 5d, 7d, 9 d subgroups ( n =6).The cardiac specimens were extracted and retained, and the levels of autophagy protein LC3 and Beclin1 were detected by immunohistochemistry and Western blotting. Results Compared to normal control group, starvation model group’ s SD rat cardiac myocyte autophagy protein LC3 and Be-clin 1 positive cell number and protein expression increased in different degree, and the differences were statistically signifi-cant ( P <0.01 or P <0.05), and there were statistically significant differences of autophagy protein LC3 and Beclin 1 posi-tive cell number and protein expression between different subgroup of starvation model group ( P <0.01 or P <0.05), and there were two peaks at 2d and 7 d.Conclusion Starvation involved in the mechanism of myocardial pathological changes of myocardial process by activation of autophagy.
Keywords：Autophagy geneBeclin 1Microtubule associated protein 1 light chainMyocardiumStarvationRats
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 719-722 )