Quantitative detection of DD3 mRNA and DD3 mRNA/D in patients with early clinical diagnosis of prostate cancer with PSA in diagnostic gray zone
LI Huifeng
WU Zhenqi
LIU Min
CHEN Pan
CHEN Ying
GUO Jianming
Abstract：Objective To study the expression of DD3 mRNA and DD3 mRNA/D in the diagnosis of prostate cancer in PSA gray zone.Methods One hundred and twelve patients with sPSA 4 -10 ng /ml of benign prostatic hyperplasia ( BPH) and 25 cases of prostate cancer ( PC) were enrolled, RT-PCR and PSA were test, B type ultrasonography and biopsy of prostate were performed.Observed the difference of the DD3 mRNA, DD3 mRNA/D, serum PSA( sPSA) , prostate punc-ture biopsy specimens of PSA ( uPSA) , total /free PSA ratio ( f/tPSA) , s/uPSA, PSA density ( PSAD) etc.parameters, and used ROC curve to analyze the diagnostic value of each parameter.Results Two groups’ patients’ age, prostate volume showed no statistical significance differences ( t =12.87, t =12.31, P >0.05);TPSA, PSAD’ s difference showed statis-tics significance ( t =7.31, t =6.89, P <0.05);PC group’s DD3 mRNA/PSA mRNA ratio were significantly higher than that of BPH group ( t =7.86, P =0.009); PC group’ s DD3 mRNA/D ratio significantly higher than BPH group ( t =7.28, P =0.05).ROC curve analysis showed that when cut-off value was 0.27, the DD3 mRNA/PSA mRNA’s curve area was 0.841 (95%CI 0.666-0.997); when cutoff value was 2.01, the curve area of DD3 mRNA/D was 0.907 (95% CI 0.790-0.869) .Conclusion DD3 mRNA content and quantitative DD3 mRNA/D detection can significantly improve the detection sensitivity of the PSA gray zone prostate cancer, has great significance for PSA gray zone prostate cancer early screening and DD3 mRNA/D has better diagnostic efficiency.
Keywords：Prostate specific antigenGray zone prostate cancerDD3 mRNA/DDD3 mRNA
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 715-718 )