Expression of NADPH oxidase in heart failure patients and its relationship with myocardium fibrosis
WANG Men-glong
LIU Jianfang
LIU Menglin
FENG Ying
YUAN Wenhui
WAN Jun
Abstract：Objective To investigate the relationship between NADPH oxidase (Nox) expression and myocardial fi-brosis in heart failure patients.Methods Using Real-time PCR to detect left ventricular myocardium of Nox mRNA expression level in 15 cases with congestive heart failure (CHF group) and 9 cases of cardiac transplantation donor (control group). Western blot was used to detect Nox and MAPKs signaling pathway phosphorylation level, malondialdehyde (MDA) kit for the detection of myocardial MDA content and Sirius red staining to observe the degree of myocardial fibrosis.Results Compared with the control group, in heart failure group, the myocardial MDA content increased significantly, (6.62 ±0.80) mmol/mg-prot and (2.23 ±0.30) mmol/mgprot respectively, the difference has statistical significance ( t =-12.947, P <0.01). Myocardial Nox4 mRNA expression levels were significantly up-regulated ( t =-3.144, P <0.01), Nox2 and its subunit expression level had no obvious change; the degree of myocardial fibrosis was significantly increased ( t =-12.947, t =-23.102, P <0.01), and phosphorylation levels of MAPKs signal pathway(p38, ERK1/2 and JNK) significantly increased ( t =-15.574, t =-12.434, t =-30.123, P <0.01).Conclusion The heart failure with myocardial oxidative stress increase may upgrade the expression of Nox4.Nox4 is involved in myocardial fibrosis by activating MAPKs pathway, and in-hibiting Nox4 may reduce myocardial oxidative stress and fibrosis in patients with heart failure.
Keywords：NADPH oxidaseHeart failureOxidative stressMyocardial fibrosis
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 675-678 )