Variation characteristics of proinflammatory cytokines in patients with prostatitis and benign prostatic hyperplasia

LI Haikuan; WANG Rui; ZHOU Zengxiang

doi:10.3969/j.issn.1671-6450.2015.05.015

Abstract：Objective To observe the variation characteristics of proinflammatory cytokines in patients with prostatic hyperplasia and prostatitis.Methods From 2012 June to 2014 January, 70 cases of hospitalized patients with disease of pros-tate, prostatic hyperplasia and prostatitis patients of 35 cases as the observation group, patients only with hyperplasia of pros-tate in 35 cases as the control group.Immunohistochemistry and immuno fluorescence quantitative PCR detection method were used for detection of proinflammatory cytokines, including interleukin 8 (IL-8), fibroblast growth factor 2 (FGF-2) and ma-trix metalloproteinase 2 ( MMP-2 ) , transforming growth factor beta ( TGF-β1 ) .And then through the immunohistochemical method to detect the tumor necrosis factor alpha (TNF-α),interleukin 6 (IL-6) and cluster of differentiation 3 (CD3) con-tent;finally, the detection results were statistical analyzed.Results The observation group’s IL-8, FGF-2 and MMP-2 pro-tein and IL-8 mRNA, FGF-2 mRNA and MMP-2 mRNA were higher than those in control group ( t =2.540 1, t =2.859 9, t =12.755 1, t =19.124 5, t =12.294 4, t =8.532 3, P <0.01);TNF-αlevel was higher than the control group but the difference was not statistically significance ( t =1.427 5, P >0.05), IL-6, CD3 level were higher than that of the control group ( t =3.942 0, t =6.988 1, P <0.01); bFGF-2 protein with moderate expression in the control group was 37.1%, which was lower than those in the observation group’ s 80%, the TGF-β1 protein moderate and above expression in the control group was 20%, which was lower than that of the observation group’s 82.8%(χ2 =2.721 8,χ2 =2.6784, P <0.01).Con-clusion The expression of proinflammatory cytokines in patients with benign prostatic hyperplasia and prostatitis was higher than patients with benign prostatic hyperplasia, prostatitis may change benign prostatic hyperplasia.