Predictive value of P16 protein on adjuvant chemotherapy effect in patients with osteosarcoma
ZHU Weimin
ZHOU Guangfu
TANG Bensen
YAN Xianke
LI Jing
Abstract：Objective To study the predictive value of P16 protein expression in osteosarcoma(OS) patients under neoadjuvantchemotherapy.Methods 40patientswithOSwereselected,allpatientsreceiveddurgadjuvantchemotherapy, paraffin embedded chips were established through the operation specimens and biopsy,immunohistochemistry staining method was used to analyze the expression of P16.P16 was positive when nuclear stained were more than 30%.The resection speci-mens of 38 patients after chemotherapy were analyzed to determine tumor necrosis percentage, when tumor necrosis was more than 90%it’ s good, when tumor necrosis was less than 90%, it’ s bad.The relationship between patient gender, age, tumor location, pathological type and tumor necrosis were analyzed using single factor and multiply factor analysis.Results The ex-pression rate of P16 was 62.5%(25/40), after treatment the median tumor necrosis rate was 95.0%,57.9%(22/38) of pa-tients were"good"at response to chemotherapy.Single factor analysis showed that:P16 positive patient’ s median necrosis rate and chemotherapy response"good"rate were higher than P16 negative patients( P <0.01).Logistic regression analysis showed that:P16 was independent predictor of chemotherapy response(OR=43.50,95% CI 2.64-708.90, P <0.01), there was no correlation between P16 expression and gender, age, tumor type, location.Conclusion The expression of P16 was significantly related to chemotherapy effect in osteosarcoma, it could be used as a marker to choice the patients in the need for chemotherapy before operation.
Keywords：OsteosarcomaChemotherapy responseP16 protein
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 369-371,379 )