In vitro study of T cell responses induced by lung cancer ’ s whole antigen pulsed with autologous dendritic cells
HUO Zhongchao?
LIU Xiaoxia
WANG Xueling
CHEN Jianhua
SU Ying
Abstract：Objective To investigate the load cell lung cancer antigen autologous dendritic ( DCs )-induced T cell proliferation and cytotoxicity in vitro. Methods Lung cancer tissue, normal lung tissue and peripheral blood of fresh speci-mens were taken from 7 patients with squamous cell carcinoma who were treated with operation, normal lung tissue and lung cancer were prepared for single cell suspension. From the peripheral blood, mononuclear cells isolated and induced DCs;ny-lon wool columns were used to isolate the T cells. Using frozen melt method to prepare the autologous lung cancer cell lysates liquid, and stimulate the DCs, preparation of loaded with autologous lung cancer antigen DCs. Using MTS method to detect DCs complete antigen load of lung cancer on T cell proliferation;flow cytometry determination of DCs phenotype and the effect of load DCs complete antigen of lung cancer on T cell cytotoxicity. Results Peripheral blood mononuclear cells through GM-CSF, IL-4 and TNF-α’ s induce, a large number of dendritic protrusions, surface molecules CD14 expression was significantly lower ( t =20. 157, P <0. 01), CD1a, CD11c, CD80, CD83 and HLA-DR expression was significantly increased ( t =8. 927, t =3. 042, t =14. 311, t =18. 537, t =5. 612, P <0. 05, P <0. 01), mature DCs which was obtain from whole load of lung cancer antigen can stimulate autologous T cell proliferation, different patients revealed different stimulation index:6. 98-39. 15 (21. 56 ± 4. 38), and the higher the DC/T ratio, the higher the stimulation index, the stronger of T cells ability to respond to the proliferation ( t =31. 203, t =19. 550, P <0. 01);DCs of load lung cancer cell whole antigen can induce cytotoxicity of autologous T cells,and T cells to autologous tumor cell killing rate was(56. 52 ± 2. 75)%;normal cell average killing rate was (5. 31 ± 1. 37)%, significantly lower than for the autologous tumor cell killing rate ( t =16. 429, P <0. 01). The killing rate of K562 cell was (9. 71 ± 2. 46)%, which is significantly lower than the killing rate of autologous tumor cells (t = 25. 010, P <0. 01). Conclusion Load lung cancer complete antigen’s DCs can induce lung cancer pa-tients’ autologous T cell proliferation and cytotoxicity, and can effectively kill tumor cells in vitro, and normal cells were not killed.
Keywords：Lung cancerWhole antigenDendritic cellsAutologous T cellsCytotoxicity
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 175-178,181 )