Study on regulating of PFT-αto the PUMA in neural stem cells transplantation after cerebral ischemia
LIU Yang
LEI Xuhui
YIN Shi
JIANG Chuanlu
Abstract：Objective To explore the effect of p 53 inhibitor ( PFT-α) on neural stem cells after cerebral ischemia in p53 downstream apoptotic gene (PUMA) expression.Methods Using SD rat to isolate NSCs, through the primary and pas-sage culture , by oxygen glucose deprivation ( OGD) treatment for 6 h, they were divided into OGD +dimethyl sulfoxide ( DM-SO) group and OGD+PFT-αgroup, changes of expression of the p 53 protein and PUMA by immunoblotting technique were compared between the 2 groups.In vivo tests using Longa method to establish the CT confirmed the brain artery embolism model in 48 rats, they were randomly divided into PFT-α+NSCs transplantation group ( n =24) and DMSO+NSCs trans-plantation group ( n =24) ;using immunofluorescence to detect the PUMA expression in the different groups of transplanta -tion.Results (1) In vitro oxygen glucose deprivation after 6 h, OGD+PFT-αgroup’s p53 protein into the nucleus level de-creased , expression is up-regulated in the cytoplasm , while OGD+DMSO group was mainly expressed in the cell nucleus;the expression level of PUMA was significantly lower than that in OGD +DMSO group ( P <0.05); (2) In vivo experiment, compared with the DMSO +NSCs group , PFT-α+NSCs PUMA transplantation group ’ s positive expression cells was signifi-cantly reduced [(38.9 ±4.3)%vs.(29.4 ±5.6)%, P <0.01].Conclusion The PFT-αcombined with neural stem cells can inhibit the expression of PUMA , which may be an important mechanism of PFT-αto promote cell survival in the cerebral ischemic area after the transplantation of neural stem .
Keywords：PFT-alphaPUMANeural stem cellsCerebral ischemiaApoptosis
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 1156-1159 )