Evaluation of ANA and autoimmune liver disease associated antibodies in diagnosis of primary biliary cirrhosis

HU Ling; GU Lei; LIU Beibei; GAO Chengcheng

doi:10.3969/j.issn.1671-6450.2014.11.007

Abstract：Objective To investigate the anti-nuclear antibody ( ANA) and autoimmune hepatitis antibodies in detec-tion of primary biliary cirrhosis .Methods From July 2012 to December 2013 , hospitalized patients with primary biliary cir-rhosis of 40 cases were enrolled as the observation group , 86 patients with non-autoimmune liver disease patients as control group .Anti-nuclear antibodies in the sera of patients were detected by indirect immunofluorescence ( ANA) , Western blot was used to detect anti mitochondrial antibody (AMA), SP100,SLA/LP,LC-1, LK-M, gp-210, analyzed the diagnostic value of them.Results In the observation group, AMA positive rate was 95.00%(38/40), significantly higher than that in the con-trol group of 5.81%(5/86) (χ2 =6.25, P <0.05), ANA’ s the centromere type, nuclear membrane and nuclear multi point detection rates were 35.00%(14/40), 30.00%(12/40) and 22.50%(9/40), significantly higher than the control group with 11.63%(10/86), 5.81%(5/86) and 3.49%(3/86) (χ2 =2.725、6.780、2.876, P <0.05).The observa-tion group’ s SP-100, SLA/LP, LC-1, LK-M, gp-210 positive rate were obviously higher than those of the control group (30.00%, 22.50%, 17.50%, 10.00%, 15.00%vs.3.49%, 1.16%, 4.65%, 2.33%, 3.49%, P <0.05).The ob-servation group’s sensitivity, specificity and coincidence rate was 92.50% (37/40), 87.50% (35/40), 95.00% (38/40), which were significantly higher than the control group with 84.88%(73/86), 77.91% (67/86), 87.21% (75/86) (P <0.05).Conclusion Clinical application of ANA and autoimmune hepatitis antibody spectrum joint detection can im -prove the detection rate of clinical primary biliary cirrhosis , provides important reference value for the diagnosis and treatment .