Effects of L-dopa on colon tyrosine hydroxylase andɑ-synuclein expression of rats model of Parkinson disease

BI Shu-li; LIU Bin; GAO Haiying

doi:10.3969/j.issn.1671-6450.2014.10.018

Abstract：Objective To investigate the effect of levodopa (L-dopa) on Parkinson's disease (PD) rats colon tyrosine hydroxylase(TH)andtheexpressionofalpha-synuclein(ɑ-Syn).Methods 72healthySDratswererandomlydividedinto control group, model of PD group (model group),L-dopa treatment group (treatment group), each group was subdivided into the successful preparation of the model after 4 d, 8 d and 2 sub-groups, 12 rats in each group.Preparation of PD model rats were performed with subcutaneous injection of rotenone , immunohistochemistry and Western blotting blot were used to detect thecolonicTHandɑ-Synexpressionlevel.Results 1hfecaloutputandwatercontent:comparedwithcontrolgroup,therats in model group and the treatment group decreased significantly ( P <0.05); compared with the model group, rats in the treatment group were increased ( P <0.05); and compared with the treatment 4 d subgroups, treatment 8 d group was in-creased ( P <0.05).(1)Compared with the control group, model group and treatment group at each time point , colon TH positive cells and protein expression were significantly reduced , the expression of ɑ-Syn positive cells and protein were signifi-cantly increased ( P <0.05);(2)Compared with the model group, the treatment group at each time point, colon TH positive cells and protein expression were increased , the expression of ɑ-Syn positive cells and protein was significantly reduced ( P <0.05);(3)Compared with the 4 d treatment group, TH positive cells and protein expression was elevated in 8 d treatment subgroups ( P <0.05),ɑ-Syn positive cells and protein were decreased ( P <0.05).Conclusion The mechanism of PD model rat colonic dysfunction may related with reduced dopamine and increased ɑ-Syn level,L-dopa may by increasing dopa-mine and reduce the level of ɑ-Syn thus improve colonic dysfunction in PD rats .