Effects of Shenshao oral liquid on MMP-2, TIMP-2 expression and myocardai l enzyme of myocardium ischemia ;repefr usion in rats
LIU Fan
DI Yali
JI Zheng
SHANG Xiaoming
Abstract：Objective To observe the effect of Shenshao oral liquid on myocardial ischemia reperfusion injury , matrix metalloproteinase-2 (MMP-2) and tissue inhibitor of matrix metalloproteinase-2 (TIMP-2)and myocardial kinase of rat model. Methods 40 SD rats were randomly divided into 4 groups, namely sham operation group (A group), ischemia reperfusion group (B group) and Shenshao oral liquid group (C group) and rosuvastatin group (D group), 10 rats in each group.C group and D group were given 3 days of Shenshao oral liquid for 8 ml/d, rosuvastatin 10 mg/d orally before operation , A group, B group received saline .By using the method of the left coronary artery ligation on rats anterior descending artery for 30 min, then reperfusion for 120 min to replicate myocardial ischemia reperfusion rats model ( sham operation without liga-tion) .Analyze rat myocardial HE staining , MMP-2 and TIMP-2 expression and myocardial kinase changes .Results Myocar-dial HE staining:A group myocardial structure was normal , the most serious damage to myocardial cells was in B group , C group ’ s myocardial cell damage was milder than that in group B and D group .Immunohistochemical detection:MMP-2 protein expression level was A group <C group <D group <B group, there was statistical significance ( P <0.05);TIMP-2 protein expression level was A group >C group >D group >B group, there was statistical significance among each group ( P <0.05).Rat myocardial enzyme: level of CK expression was very small in A group , the other groups were expressed a lot (P <0.05); CK-MB, LDH level: A group <C group <D group <B group, there were statistical significance ( P <0.05).Conclusion The Shenshao oral liquid preconditioning on myocardial ischemia reperfusion injury in rats has a protec -tive effect, the mechanism may be related to the decrease of MMP-2 and increase the expression of TIMP-2.
Keywords：Shenshao oral liquidIschemia reperfusionMatrix metalloproteinase 2Tissue inhibitor of metallopro-teinase 2Rets
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 926-928,931 )