Effect of batimastat on hypertension mice and its serum matrix metalloproteinase 9
LI Hui
PENG Lijing
LIU Wei
YE Mingxia
TUO Buxiong
Abstract：Objective To study the effect of matrix metalloproteinase 9 ( MMP-9) inhibitor batimastat on blood pres-sure, serum MMP-9 level and activity in hypertension mice .Methods Thirty-six hypertension ( hypertension group ) mice were divided into 3 groups, with 12 rats in each group, give 3 days of high dose of batimastat (30 mg/kg, high dose group), low dose of batimastat (10 mg/kg, low dose group) and physiological saline (control group) injection on mouse peritoneal in the three groups respectively , the serum levels of MMP-9 and mRNA levels were detected followed in the 1 last after injection 0 h, 2 h, 4 h, 6 h; serum levels of MMP-9 activity were detected by gelatin zymography assay .Select 36 healthy mice as healthy control group , serum levels of MMP-9 and mRNA levels were detected , and compared with hypertension group .Re-sults (1) Blood pressure change:compared with health control group , hypertension group before treatment blood pressure is higher, the difference was statistically significant ( P <0.05), after treatment, compared with the control group and low dose group, high dose group blood pressure decreased , the differences were statistically significant ( P <0.05).(2) The serum concentration of MMP-9: compared with health control group , before treatment , in the hypertension group , MMP-9 protein levels were significantly higher ( P <0.01), after treatment, the within group comparisons:in high dose group and low dose group, the expression of MMP-9 protein in the serum of mice were decreased along with prolonging the time of drugs , the differences were statistically significant ( P <0.05, P <0.01);compared with control group and low dose sub group , high dose group's serum MMP-9 concentration was low at 2 h,4 h,6 h,the differences were statistically significant ( P <0.05, P <0.01);compared between low dose group and control group , 2 h, 4 h, 6 h the difference had statistical significance ( P <0.05).(3) The expression of MMP-9 mRNA: compared with health control group , hypertension group MMP-9 mRNA was elevated ( P <0.05);after treatment, high dose group and low dose group of mice'level of serum MMP-9 mRNA were de-creased with the increase of drug action time , differences were statistical significance ( P <0.05, P <0.01);paired compas-sion of low dose sub group, high dose sub group, control sub group showed significant differences ( P <0.05, P <0.01). (4) Determination of MMP-9 activity:with the batimastat dose increased, Pro MMP-9 and Active MMP-9 activity decreased. Conclusion Batimastat can reduce blood pressure level and serum MMP-9 level and activity of high blood pressure mice .
Keywords：BatimastatMatrix metalloproteinase 9HypertensionMice
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 724-727 )