Influences of ischemic preconditioning on autophagy in rats with cerebral ischemia reperfusion injury
SHI Qiuyan
LIU Bing
ZHANG Chunyang
FAN Yaxia
SUN Yuan
YANG Bin
Abstract：Objective To observe the effect of ischemic preconditioning ( IPC) effect on cerebral ischemia reperfusion in rat hippocampus of autophagy .Methods According to method of Zea-Longa filamene , rats middle cerebral artery ischemi-a-reperfusion model were established , the experimental animal were randomly divided into 3 groups: sham operation group (Sham group, n =10), ischemia reperfusion group (I/R group, n =10), IPC treatment group (IPC group, n =30), in IPC group, according to ischemic preconditioning time , rats were divided into 1 d, 3 d, 5 d subgroups, 10 rats in each group.Af-ter ischemia 2 h and reperfusion 24 h, immunohistochemical method was used to detect the autophagy associated protein BEC -LIN L, LC3-II expression, transmission electron microscope in nerve cell autophagy and lysosomal activation and cell ultra -structure changes .Results ( 1 ) Neurological dysfunction score: compared with Sham group , I/R group and IPC group showed varying degrees of nerve dysfunction ( P <0.01), I/R group of symptoms in IPC subgroups ( P <0.05).(2) The volume of cerebral infarction:compared with Sham group , I/R group and IPC group had different degree of infarction ( P <0.01);IPC group was lower than that in I/R group ( P <0.01), 3 d subgroup was less than 1 d and 5 d subgroup ( P <0.01).(3) Immunohistochemical staining:compared with Sham group , I/R group and IPC group, the number of BECLIN 1 and LC3-II positive cells ( P <0.01), the expression of IPC positive cells was significantly lower than that of I /R group (P <0.01), IPC 3 d subgroup was less than 1 d, 5 d subgroup ( P <0.01).(4) The change of brain tissue ultrastructure morphology of cells:Sham group was normal;I/R group and IPC group showed varying amounts of autophagy , in different peri-od and or autophagic lysosome , mitochondria swelling, rupture of membranes, vesicles, lysosomes increased significantly ,visi-ble deformation of secondary lysosomes ,Golgi fragmentation;IPC group autophagy and the number of lysosomes was significantly reduced compared with I/R, the number of the IPC between the subgroups of autophagosomes and lysosomes revealed no visible change.Conclusion IPC may inhibit autophagy relieve the ischemic reperfusion injury ,3 d IPC is better than 1 d, 5 d.
Keywords：AutophagyIschemic preconditioningIschemia reperfusion injuryBECLIN 1LC3-II
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 721-723,727 )