Effects of preconditioning with exendin-4 on myocardial ischemia/reperfusion injury in rats
LU Jiajia
HU Xiaorong
HU Gangying
JIANG Hong
Abstract：Objective To investigate the effect of exendin 4 ( Ex-4) preconditioning on rat ischemia/reperfusion ( I/R) damage.Methods Fifty-five male SD rats were randomly divided into 4 groups:sham operation group (SO) ( n =10), ischemia /reperfusion (I/R) group ( n =15),EX4+I/R (Ex-4-I/R) group (n=15), Ex-4+exendin (9-39)[Ex-4-Ex (9-39)-I/R] group ( n =15).Except SO group, the other 3 group rats underwent left anterior descending coronary artery ligation, after 30 min ischemia, reperfusion of 4 h, Ex-4-I/R group was given Ex-4 before I/R pretreatment, Ex-4-Ex(9-39)-I/R group was given Ex-4 and Ex-(9-39) preconditioning before I/R group.After reperfusion for 4h, then assessed of the my-ocardial infarction area , and get the carotid artery blood sample to test the myocardial enzyme , myocardial oxidative index , myocardial high mobility group protein 1 (HMGB1) levels.Results 4h after reperfusion, compared with group I/R, Ex-4-I/R group reduced myocardial infarct size more obviously , (54.1 ±3)% and (25.8 ±1)% respectively, the difference was statistically significant ( P <0.05) compared with Ex-4-I/R group, Ex-4-Ex(9-39)-I/R group myocardial infarction area in-creases, as of (47.8 ±3.0)%,the difference was statistically significant ( P <0.05).Compared with SO group, I/R group's LDH, CK, MDA levels were significantly increased, HMGB1 expression was significantly increased ( P <0.05), and SOD level decreased significantly ( P <0.05);compared with I/R group, Ex-4-I/R group's indicators improved significantly , the difference was statistically significant ( P <0.05);compared with the Ex-4-I/R group, Ex-4-Ex(9-39)-I/R group's indica-tors improvement were suppressed , the difference was statistically significant ( P <0.05).Conclusion It demonstrated that the Ex-4 preconditioning may inhibit the expression of HMGB 1 and reduce the myocardial I/R injury in rats .
Keywords：exendin-4Myocardial ischemia/reperfusion injuryHigh mobility group protein 1Rats
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 717-720 )