Inhibition effects of SAHA on human cervical cancer cells in nude mice xenografts
FU Xinxin
TIAN Zhihua
HAO Ganghua
LIU Zhanwen
LIU Jin
XING Jun
Abstract：Objective To observe the tumor growth inhibition effect of SAHA to human cervical cancer transplanted in nude mice and to discuss the mechanism .Methods Cultured human cancer cell lines in nude mice inoculated to 20 dorsal roots of the right lower limb subcutaneous , 15 d after the tumor will become 18 mice were randomly divided into three groups with each included 6 mice:blank control group, SAHA1 group (25 mg/kg), SAHA2 group (50 mg/kg).Consecutive 4d in-traperitoneal injection of drugs , intermittent 7 d, and then continuously administered 4 d, 1 times/d.Observe the growth of cancer xenografts in nude mice in each group , the inhibition rate was calculated .Immunohistochemistry and Western blot a-nalysis was used to detect the expression of tumor in each group Bax and Bcl-2 protein.Results Tumor suppression in blank control group was 0, SAHA1 group was 22.44%, SAHA2 group was 35.52% , the two groups was statistically significant difference ( P <0.05).After treatment, SAHA2 group nude body mass were lower than the blank control group and SAHA 1 group ( P <0.05).After treatment, SAHA1 group SAHA2 tumor volume was less than the blank control group , and the SA-HA2 group was less than SAHA1 group( P <0.05).Compared with the blank control group , SAHA1 group and SAHA2 nude mice's tumors with low quality, and SAHA2 group lower than SAHA1 group, the differences were statistically significant (P <0.05);in immunohistochemistry test and protein blotting method , compared with blank control group , tumor tissue Bax protein in SAHA1 group and SAHA2 group were significantly increased , Bcl-2 protein were significantly lowered ( P <0.05).the changes in SAHA2 group were more obvious than SAHA1 group ( P <0.05).Conclusion SAHA can inhibit human cancer xenograft SiHa cell growth , Bax/Bcl-2 -related apoptosis is one of the tumor suppressor mechanism .
Keywords：Suberoylanilide hydroxamic acidCervical cancerNude miceBcl-2Bax
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 399-402 )