miR-126 expression and its effect on human colon cancer cells in the biological behavior of colon cancer cells

LIN Mengbo; WAGN Jinsi; XUE Fangqin; HUANG Ruolei; LI Weihua

doi:10.3969/j.issn.1671-6450.2014.04.021

Abstract：Objective To investigate the expression of MicroRNA -126 (miR-126) in human colon cancer cells and its effect on biological behavior of colon cancer cells .Methods Sixty patients with colon cancer were enrolled in the study . Fluorescence PCR was used to detect the expression of miR-126 in the cancerous tissues and in the tissues adjacent to carcino-ma.The expression of miR-126 in the cancerous tissues paired up with the expression of miR-126 in the tissues adjacent to carcinoma, and there were 60 pairs in total.The expression of miR-126 in 5 colon cancer cell lines ( SW480, HT29, HCT116, LOVO, SW620) was also detected.The proliferation, migration and invasion ability of the cells were measured u-sing CCK 8 assay, scratch experiments and Transwell assay , respectively.The effect of miR-126 on the biological behaviors of human colon cancer cells was further studied through the experiments in vitro .Results Compared with adjacent normal tissue cells, miR-126 was decreased (95.0%vs.55.0%, P <0.05) in colon cancer cells , and were obviously when cancer metas-tasis or invasion is particularly evident ,when compared with those without metasis ,there were significant difference (25.0%vs.75.0%, P <0.05).Studies have shown that miR-126 can inhibit the growth of SW480 cells and their invasion and metas-tasis, Transwell assay with matrigel showed the migration cell number in transfection group and blank control group was (10.0 ±4.3) and (258.0 ±30.6),while Transwell assay without matrigel showed the migration cell number was (84.0 ± 13.2) and (335.0 ±27.3) respectively,there were significant differences( P <0.05).miR-126 expression can enhance the sensitivity of cancer cells to oxaliplatin .Conclusion miR-126 can inhibit the development of colon cancer cells , the impact of a variety of strains of the biological behavior of colon cancer cells .