Evalutation of In Vitro Antibacterial Activity of Four Tibetan Medicinal Plants from the Ranunculaceae Family
Xie Linglin
Yin Min
Liu Ping
Abstract：Objective To investigate the in vitro antibacterial activities of four Tibetan medicinal plants from the Ra-nunculaceae family(Delphinium brunonianum Royle,Delphinium trichophorum Franch,Delphinium caeruleum Jac-quem.ex Cambess and Clematis florida Thunb)against six pathogens.Methods The 85%ethanol extracts of four Tibetan medicinal plants from the Ranunculaceae familywere subjected to sequential extraction with ethyl acetate,n-butanol,petroleum ether and water,followed by concentration and freeze-drying to obtain four polar components.The in vitro antibacterial activities of each component were evaulated against Staphylococcus aureus,Enterococcus faecalis,Sarcina lutea,Escherichia coli,Pseudomonas aeruginosa and Klebsiella pneumoniae using the microdilu-tion method.Results The in vitro antibacterial results showed that the petroleum ether part of Delphinium caerule-um Jacquem.ex Cambess,Delphinium brunonianum Royle and Clematis florida Thunb exhibited the best inhibitory effect on Staphylococcus aureus,with average minimum inhibitory concentration(MIC)value of 3.125 mg/mL.The ethyl acetate and petroleum ether parts of Delphinium caeruleum Jacquem.ex Cambess and,the n-butanol part of Clematis florida Thunb showed the best inhibitory effect on Enterococcus faecalis,with average MIC value of 3.125 mg/mL.The ethyl acetate and petroleum ether parts of Delphinium caeruleum Jacquem.ex Cambess,petro-leum ether parts of Delphinium brunonianum Royle and Clematis florida Thunb showed the best inhibitory effect on Sarcina lutea,with average MIC value of 3.125 mg/mL.The ethyl acetate and n-butanol parts of Clematis florida Thunb showed the best inhibitory effect on Escherichia coli,with average MIC value of 6.25 mg/mL Conclusion The four selected Tibetan medicinal plants demonstrated better inhibitory activities against Gram-positive bacteria than Gram-negative bacteria,among which,Delphinium caeruleum Jacquem.ex Cambess exhibited the best antibacteri-al effect,suggesting its potential for further research.
Keywords：Tibetan medicinePlants from the Ranunculaceae familyMinimum inhibitory concentration(MIC)In vitro antibacterial activities
Publication Date：2025-06-30
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 58-63 )