Analysis of the community composition and diversity of endophytic fungi and soil fungi in different organs of Rhodiola crenulata in Mila Mountain,Xizang

Zhang Junze; Mao Kangshan; Suo Nancuo

doi:10.3969/j.issn.1004-3403.2024.04.006

Abstract：Rhodiola crenulata,a perennial herb,is an endangered Tibetan medicinal plant with antioxidant,anti-al-pine hypoxia,anti-fatigue,liver protection,hypoglycemia,anti-radiation and other effects.Plant endophytes live in healthy plant tissues,can synthesize substances needed for plant growth,induce host plant disease resistance,and en-hance plant resistance to environmental stress.As an important part of plant ecosystems,rhizosphere microorganisms play a pivotal role in nutrient acquisition,stress tolerance and material cycling in the process of plant growth and de-velopment.Therefore,the study of endophytic fungi and rhizosphere fungi of Rhodiola crenulata is of great signifi-cance for the development and protection of this species,as well as for the development and protection of endangered Tibetan medicinal plant resources on the Tibetan Plateau.In this study,Illumina MiSeq high-throughput sequencing was used to analyze the composition and diversity of fungal communities in the ITS1 region of Rhodiola crenulata en-dophytic(roots,stems,leaves),rhizosphere,and root-zone fungi in Mila Mountain,Xizang.The results showed that at the genus level,the dominant genus was minutisphaera in roots,lemonniera in stems,lemonniera in leaves,helotia-les in rhizosphere soil,and helotiales in root zone.The diversity of endophytic fungi in the stems of Rhodiola crenula-ta was significantly higher than that in roots and leaves.The study systematically revealed the community characteris-tics of the endophytic fungi and rhizosphere fungi of Rhodiola crenulata,and provided a reference for screening the active strains of Rhodiola crenulata and the interactions between Rhodiola crenulata and endophytic fungi.