Research on the rapid propagation system of the Tissue Culture of Gentiana urnula
Lu Yuedong
Ni Zhen
Yangjin Lamu
Abstract：In order to establish the rapid propagation system of Gentiana urnula,the research selected Gentiana urnula plants collected from Yangda Village,Duilong Deqing District,Lhasa,Xizang Autonomous Region for the rapid propa-gation experiment of tissue culture.The most suitable explants,the optimal disinfection time of HgCl2 solution,the opti-mal adventitious bud inducd differentiation medium,and the best rooting culture medium for Gentiana urnula were screened.The results showed that the stem segment with nodes was suitable as an explant in the tissue culture experi-ment of Gentiana urnula,while the leaf block was not suitable as explants.The optimal disinfection time for HgCl2 so-lution is 6 minutes,with an average uncontaminated rate of 66.67％±3.33％and an average survival rate of 63.33％± 3.33％.The optimal adventitious bud induced differentiation medium was as follows of P7 treatment group(MS+0.1g/L inositol+30g/L sucrose+5g/L agar powder+2.0mg/L 6-BA+1.0mg/L IAA pH 5.6),with an average bud proliferation multiple of 2.47±0.12,an average bud length of 17.07±1.56mm,and an average unvitrified rate of 80.00±5.57％.The optimal rooting culture medium was as follows ofT5 treatment group(1/2MS+0.1g/L inositol+30g/L sucrose+5g/L agar powder+0.3mg/L NAA+0.3mg/L IBA pH 5.6),with an average rooting rate of 100％,an average rooting number of 3.63±0.09,an average rooting length of 39.03±0.30mm,and an average rooting health rate of 85.27％±1.22％.
Keywords：Gentiana urnulatissue culturerapid propagation
Publication Date：2024-01-28
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：7( 12-18 )