Biological Activities of Phenolic Compounds Extracted and Purified from Burdock Root
LI Chao
HOU Kaige
QIAO Yuxin
GU Lingling
LIU Yongxin
Abstract：To investigate the active ingredient contents,antioxidant,antibacterial,and hypolipidemic activities of phenolic compounds from burdock root,crude extract(CE)was obtained using 60％ ethanol extraction.This was purified with AB-8 macroporous resin to yield F60,followed by further purification using D101 macroporous resin with 20％,40％,and 60％ethanol gradient elution to obtain F60-20,F60-40,and F60-60,respectively.Spectrophotometric methods determined the contents of total phenolics,total tannins,total flavonoids,and total phenolic acids.Antioxidant activity was evaluated through free radical scavenging(DPPH,ABTS+)and metal ion reduction(molybdenum,copper)assays.Antibacterial activity was assessed via microplate dilution method,while hypolipidemic activity was evaluated by lipase inhibition analysis.Experimental results indicated that F60-60 and F60-40 exhibited higher levels of total phenolics,tannins,flavonoids,and phenolic acids among all samples.These fractions demonstrated effective DPPH and ABTS+radical scavenging capacities,significant molybdenum/copper ion reduction capabilities,and notable lipase inhibition activity.CE showed inhibitory effects against Listeria monocytogenes;F60,F60-20,and F60-60 against Salmonella;and F60-40 against Bacillus cereus.
Keywords：burdock rootphenolic compoundsethanol extractpurified fractionantioxidant activityantibacterial activityhypolipidemic activity
Publication Date：2025-06-30
Online Publishing Date：2025-08-26(First online date of this platform, not the publication date of the document)
Pages：7( 43-49 )