On the Extraction Technique of Optimizing Ginkgo Isoflavone Based on Response Surface Methodology
GAO Shu-yun
Abstract：This paper ,with ginkgo nut as raw material ,synthesized the Soxhlet extraction time ,solid‐liquid ratio ,ethanol concentration ,extraction temperature and single factor experiment to determine the op‐timal level of single factor and to do the response surface optimization experiments .Finally the optimum technological conditions for extracting the isoflavone compounds with ethanol as extracting agent had been obtained .Then the isoflavone content was also determined by the ultraviolet spectrophotometric method . The results showed that the best optimum extraction process conforms to the following conditions :soxhlet extraction time 5 h ,solid‐liquid ratio 1 ∶ 30 ,80% ethanol concentration ,extraction temperature of 97 .45DEG c .After being measured by ultraviolet spectrophotometer ,the recovery rate is 98 .15% ,the pre‐cision is 3 .391% ,ginkgo fruit isoflavone content is 2 .255 mg/g .
Keywords：ginkgo fruitextractionisoflavoneultraviolet spectrophotometryresponse surface opti-mization experiment
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：7( 32-38 )