Supercritical Carbon Dioxide Extraction of Oil from Litsea Cubeba and the Antioxidant Activity Test of the Oil
Abstract：Extraction of volatile oils from the dry fruits of Litsea cubeba with supercritical carbon dioxide was studied. The influence of temperature, pressure, CO2 flow rate and time on the efficiency of extraction was examined by orthogonal experiment. Meantime, the antioxidant activity of Litsea cubeba oil was measured by the DPPH test, and it was studied for the first time bow the application of co-solvent and the extraction time can affect the rate of extraction and the oil＇s antioxidant activity. The results show that the optimal extracting conditions are determined as pressure 25 MPa, temperature 50℃, CO2 flow rate 40 L/h, with largest extraction rate of volatile oils as 36.7 %. The extraction time can make big difference on that from the first 20 min. It is proved that adding the co-solvent can improve extract＇s total yield and antioxidant activity. The extraction rates can reach 45.88 % when acetic ether used as co-solvent. The best antioxidant activity can be achieved when methyl alcohol is used as co-solvent, with the IC50 measured by DPPH method as low as 0.30 g/L, compared with 7.63 g/L of CK.
Keywords：Litsea cubebasupercritical carbon dioxide extractionantioxidant activity testco-solvent
Publication Date：2012-04-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 16-20 )