Identification through 16S rDNA Sequence Analysis and Marking with Green Fluorescent Protein of an Isolated Strain for Microbial Enhanced Oil Recovery
Abstract：A strain（named YX） isolated from Shengli Oil Field,capable of producing polysaccharides for Microbial Enhanced Oil Recovery（MEOR） ,has been identified as a member of Enterobacter sp.through 16SrDNA sequence analysis.Recombinant plasmid of pET-30a（＋） -EGFP has been transferred into natural strain YX using cold CaCl2 method,and green fluorescent expressed by engineered strain YX containing green fluorescent protein（GFP） gene has been observed and monitored.This study provides a new approach that can track and monitor the dynamic data of a goal strain for enhanced oil recovery.
Keywords：Microbial Enhanced Oil Recovery（MEOR） strain16S rDNAgreen fluorescent protein（GFP）recombinant plasmid
Publication Date：2011-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 56-60 )