Progress on single-cell sequencing technology in central nervous system autoimmune diseases
WENG Shi-wen
LI Cheng-yu
SHAN Wei
WANG Qun
Abstract：Central nervous system(CNS)autoimmune diseases are characterized by complex pathogenesis involving interactions among diverse immune cells originating from both the CNS and the peripheral immune system.Single-cell sequencing technology enables high-resolution expression profiling at the single-cell level,allowing precise characterization of cellular heterogeneity and functional phenotypic dynamic.In recent years,this technology has been increasingly applied to the study of CNS autoimmune diseases,including multiple sclerosis(MS),neuromyelitis optica spectrum disorders(NMOSDs),autoimmune encephalitis(AE),and myasthenia gravis(MG).Single-cell sequencing technology has shown unique advantage in elucidating the immunopathogenesis of disease,identifying pathogenic immune cell subsets,elucidating clonal evolution patterns,and facilitating the discovery of potential biomarkers and therapeutic targets.This review summarizes the development of single-cell sequencing technology and highlights the applications in CNS autoimmune diseases,with an emphasis on the implications for advancing the understanding of disease mechanisms and supporting precision diagnosis and targeted therapeutic strategies.
Keywords：Autoimmune diseases of the nervous systemCentral nervous systemCytological techniquesReview
Publication Date：2025-12-25
Online Publishing Date：2026-01-23(First online date of this platform, not the publication date of the document)
Pages：6( 1107-1112 )