Magnetocaloric antitumor and osteogenic properties of magnetic bioactive glass scaffolds

Li Qingshan; Li Runmeng; Gao Yuyang; Han Gang; Chen Jiying; Guo Quanyi

doi:10.12307/2026.630

Abstract：BACKGROUND:With the development of tissue engineering,it is of great significance to design a bioactive scaffold that prevents tumor recurrence and possesses good osteogenic properties. OBJECTIVE:To evaluate the biocompatibility,magnetic thermal antitumor properties,and osteogenic differentiation potential of borosilicate bioactive glass scaffolds loaded with magnetic hydrogels. METHODS:Magnetic hydrogels were prepared by combining methacrylated gelatin with magnetic ferric oxide nanoparticles and were loaded onto borosilicate bioactive glass scaffolds to create magnetic bioactive glass scaffolds.The morphology and magnetothermal properties of the magnetic bioactive glass scaffolds were characterized.Rat bone marrow mesenchymal stem cells were cultured with borosilicate bioactive glass scaffolds and magnetic bioactive glass scaffold extracts,respectively.The biocompatibility of the scaffolds was evaluated by CCK-8 assay and live/death staining.After osteogenic induction,the osteogenic properties of the scaffolds were evaluated by alkaline phosphatase staining,alizarin red staining,and qRT-PCR detection of osteogenic gene expression.Human osteosarcoma cells were co-cultured with borosilicate bioactive glass scaffolds,magnetic bioactive glass scaffolds,and magnetic bioactive glass scaffolds under alternating magnetic field intervention.The anti-tumor properties of the scaffolds were evaluated by CCK-8 assay,live/death staining,and flow cytometry apoptosis detection. RESULTS AND CONCLUSION:(1)Scanning electron microscopy showed that the hydrogel carrying magnetic nanoparticles was stably loaded in the pores of the borosilicate bioactive glass scaffold.The hysteresis and magnetic thermal curves indicated that the magnetic bioactive glass scaffolds exhibited good magnetic thermal properties.(2)CCK-8 assay and live/dead staining the magnetic bioactive glass scaffold and the borosilicate bioactive glass scaffold had no obvious cytotoxicity and both promoted the proliferation of rat bone marrow mesenchymal stem cells,among which the magnetic bioactive glass scaffold had a more obvious promoting effect.Under osteogenic induction conditions,both groups of scaffolds could promote the osteogenic differentiation of rat bone marrow mesenchymal stem cells and increase the mRNA expression of osteocalcin and Runx2,among which the magnetic bioactive glass scaffold had a more obvious promoting effect.(3)CCK-8 assay,live/death staining,and flow cytometry apoptosis detection showed that compared with the borosilicate bioactive glass scaffold and the magnetic bioactive glass scaffold,the magnetic bioactive glass scaffold under the intervention of alternating magnetic field could inhibit the proliferation activity of human osteosarcoma cells and induce cell apoptosis.The results showed that the magnetic bioactive glass scaffold had good magnetocaloric properties,osteogenic properties and anti-tumor properties.