Chemokine receptor 7-bone marrow mesenchymal stem cells combined with porcine small intestinal submucosa promote skin repair in rats

Fan Meirong; Li Guangqi; Song Xumei; Yan Xin; Sui Ruizhi

doi:10.12307/2026.058

Abstract：BACKGROUND:The clinical outcomes of autologous and allogeneic skin transplants,which are commonly used for repairing skin lesions,are often suboptimal.In recent years,advancements in tissue engineering have provided new hope for skin repair.Nevertheless,the regeneration of blood vessels within skin tissue engineering remains a significant challenge.Porcine small intestinal submucosa and bone marrow-derived mesenchymal stem cells are widely utilized natural extracellular matrix biomaterials and seed cells in current tissue engineering research.Chemokine receptor 7 is a cytokine that can promote angiogenesis. OBJECTIVE:To observe the repair effect and angiogenesis ability of chemokine receptor 7-bone marrow mesenchymal stem cells-porcine small intestinal submucosa membrane on rat back skin damage. METHODS:(1)The adenovirus vector overexpressing chemokine receptor 7 was used to transfect bone marrow mesenchymal stem cells.The transfection efficiency was evaluated using western blot assay and RT-qPCR.(2)The bone marrow mesenchymal stem cells overexpressing chemokine receptor 7 were co-cultured with porcine small intestinal submucosa.Cytocompatibility was assessed through scanning electron microscopy and live/dead cell staining.(3)12 SD rats were utilized to establish a skin defect animal experimental model,and chemokine receptor 7-bone marrow mesenchymal stem cells-porcine small intestinal submucosa(experimental group)and porcine small intestinal submucosa(control group)were placed in the rat skin defect,and the wound healing was observed 1,3,7,and 14 days after modeling.The expression of vascular endothelial growth factor protein in the wound healing tissue was detected by western blot assay at 7 and 14 days after modeling,and the expression of CD31 and proliferating cell nuclear antigen protein in the wound tissue was detected by immunohistochemical staining. RESULTS AND CONCLUSION:(1)Bone marrow mesenchymal stem cells overexpressing chemokine receptor 7 were successfully constructed by adenovirus-mediated transfection.The protein and mRNA expressions of chemokine receptor 7 in the chemokine receptor 7 transfected group were significantly upregulated compared with those in the control group and the empty vector group(P<0.001).(2)Scanning electron microscopy observation and live-dead cell staining results showed that bone marrow mesenchymal stem cells overexpressing chemokine receptor 7 grew well on the surface of the submucosal membrane of the porcine small intestine,and the two had good compatibility.(3)Compared with the control group,the wound area of the experimental group was significantly reduced(P<0.05).The protein expression levels of vascular endothelial growth factor,CD31,and proliferating cell nuclear antigen in the wound healing tissue of the experimental group were higher than those in the control group(P<0.05),indicating that the ability of chemokine receptor 7-bone marrow mesenchymal stem cells-porcine small intestinal submucosal membrane to promote wound angiogenesis was strong.