Optimization of prescription ratio of"Honghuangbai"gel by orthogonal test in diabetic skin wound mouse models

Gao Yanguo; Guo Xu; Li Xiaohan; Chen Shiqi; Zhu Haitao; Huang Liangyong; Ye Fang; Lu Wei; Wang Qibin; Zheng Tao; Chen Li

doi:10.12307/2026.560

Abstract：BACKGROUND:External treatment of Chinese medicine has a long history in promoting wound healing,and it is of great significance to develop effective Chinese medicine external preparations to treat diabetic foot ulcers.The medicinal materials of the formula"Honghuangbai(Rhodiola crenulata,Phellodendron,and Bletilla striata)"have the effects of removing dead tissue and promoting tissue regeneration,promoting blood circulation and unblocking collaterals,and detoxifying and curing sores,which can effectively promote the healing of diabetic foot ulcers.However,in actual application,there are defects such as easy stratification after long-term storage and difficult cleaning during dressing changes. OBJECTIVE:To optimize the prescription ratio of"Honghuangbai"gel and develop a gel-type preparation with good stability and the effect of promoting the healing of diabetic foot ulcers. METHODS:A diabetic model of C57BL/6J mice was established by intraperitoneal injection of streptozotocin.Four weeks after successful modeling,a full-thickness skin defect with a diameter of 8 mm was made on the back of the mice.The dosage of the medicinal materials in the"Honghuangbai"gel formula(6-15 g of Bletilla striata,3-6 g of Rhodiola crenulata and 3-12 g of Phellodendron)was used as the investigation factor.The wound healing rate and its area under the curve were used as evaluation indicators to carry out an L9(34)orthogonal experiment to optimize the formulation of the"Honghuangbai"gel.Thirty C57BL/6J mice were randomly divided into a normal control group,a model control group,and an optimal prescription ratio drug treatment group(three parallel groups),with 6 mice in each group.Full-thickness skin defects were made in the normal control group;diabetic full-thickness skin defects were made in the model control group;diabetic full-thickness skin defects were made in the optimal prescription ratio drug treatment group.After 3 days of wound modeling,sterile water was applied to the wounds of the normal control group and the model control group,and the optimal prescription ratio"Honghuangbai"gel was applied to the wounds of the optimal prescription ratio drug treatment group,once a day,for 12 consecutive days.The wound healing rate and its area under the curve were used as evaluation indicators to verify the orthogonal experimental results. RESULTS AND CONCLUSION:The orthogonal test results showed that the optimal level of each factor was 6 g of Bletilla striata,4.5 g of Rhodiola crenulata,and 12 g of Phellodendron.The results of the verification experiment showed that the wound healing rate and its area under the curve of the mice in the optimal prescription ratio drug treatment group on days 4 and 6 were higher than those in the model control group.The results showed that the optimal prescription ratio of the"Honghuangbai"gel was 6 g of Bletilla striata,4.5 g of Rhodiola crenulata,and 12 g of Phellodendron,which could effectively promote the healing of skin wounds in diabetic mice.