Spatiotemporal expression of bone morphologic protein 7 in mouse kidney development

Sun Yajie; Zhao Xinchen; Bo Shuangling

doi:10.12307/2026.036

Abstract：BACKGROUND:Bone morphogenetic protein 7 is mainly synthesized in the kidney and is expressed at different stages of kidney development,but the spatiotemporal distribution of its expression and its relationship with kidney development have not been systematically reported. OBJECTIVE:To observe the expression of bone morphogenetic protein 7 in mice kidneys during different developmental stages and to explore its potential relationship with kidney development. METHODS:Immunohistochemical techniques combined with stereological methods and western blot analysis were used to observe and analyze the expression of bone morphogenetic protein 7 in mouse kidney tissues at embryonic days(E)12,14,16,18,and neonatal days(N)1,3,7,14,24,40,and 70. RESULTS AND CONCLUSION:(1)The results of immunohistochemistry showed that bone morphogenetic protein 7 was mainly expressed in the nephrogenic zone and immature renal corpuscles during the early stage of kidney development.Subsequently,it was located in mature renal corpuscles and the surrounding distal convoluted tubules,and the expression range gradually increased.After N40 days,bone morphogenetic protein 7 positively expressed in the renal interstitium.(2)Stereology and western blot results showed that the expression level of bone morphogenetic protein 7 gradually decreased with the development of the kidney,reached the lowest level at N7 days,and then slowly increased.To conclude,during the process of kidney development,the expression of bone morphogenetic protein 7 has significant spatiotemporal specificity,and it is hypothesized that it may be related to the development and maturation of renal corpuscles and distal convoluted tubules.